biostudies-literaturePrajapati JGSBTM47https://www.ebi.ac.uk/biostudies/studies/S-EPMC9764306biostudies-literatureUnknown80(1)Mucormycosis, also known as Zygomycosis, is a disease caused by invasive fungi, predominantly Rhizopus species belonging to the Order of Mucorales. Seeing from the chemistry perspective, heterocyclic compounds with an "azole" moiety are widely employed as antifungal agent for minimising the effect of mucormycosis as a prescribed treatment. These azoles serve as non-competitive inhibitors of fungal CYP51B by predominantly binding to its heme moiety, rendering its inhibition. However, long-term usage and abuse of azoles as antifungal medicines has resulted in drug resistance among certain fungal pathogens. Hence, there is an unmet need to find alternative therapeutic compounds. In present study, we used various in vitro tests to investigate the antifungal activity of eugenol against R. oryzaCurrent microbiologyA Comprehensive in vitro and in silico Assessment on Inhibition of CYP51B and Ergosterol Biosynthesis by Eugenol in Rhizopus oryzae.PMC9764306GSBTM/JD(R&D)/610/20–21/365-371GSBTM/JD(R&D)/610/20-21/365-371GSBTM/JD(R&D)/616/21–22/1236GSBTM/JD(R&D)/616/21-22/1236Rao PGoswami DAcharya DPatel SKPoojara LPrajapati JRawal RMfalseA Comprehensive in vitro and in silico Assessment on Inhibition of CYP51B and Ergosterol Biosynthesis by Eugenol in Rhizopus oryzae.Mucormycosis, also known as Zygomycosis, is a disease caused by invasive fungi, predominantly Rhizopus species belonging to the Order of Mucorales. Seeing from the chemistry perspective, heterocyclic compounds with an "azole" moiety are widely employed as antifungal agent for minimising the effect of mucormycosis as a prescribed treatment. These azoles serve as non-competitive inhibitors of fungal CYP51B by predominantly binding to its heme moiety, rendering its inhibition. However, long-term usage and abuse of azoles as antifungal medicines has resulted in drug resistance among certain fungal pathogens. Hence, there is an unmet need to find alternative therapeutic compounds. In present study, we used various in vitro tests to investigate the antifungal activity of eugenol against R. oryza2022-01-01T00:00:00Z2022 Dec2025-04-03T22:35:30.651Z2024-10-16T04:05:22.349ZS-EPMC97643063653813310.1007/s00284-022-03108-9