biostudies-literatureSevinc KCancer Research UKVersus ArthritisMedical Research CouncilAcademy of Medical Sciences2629-2642https://www.ebi.ac.uk/biostudies/studies/S-EPMC9768578biostudies-literatureUnknown17(12)Epigenetic reprogramming to pluripotency requires extensive remodeling of chromatin landscapes to silence existing cell-type-specific genes and activate pluripotency genes. ATP-dependent chromatin remodeling complexes are important regulators of chromatin structure and gene expression; however, the role of recently identified Bromodomain-containing protein 9 (BRD9) and the associated non-canonical BRG1-associated factors (ncBAF) complex in reprogramming remains unknown. Here, we show that genetic or chemical inhibition of BRD9, as well as ncBAF complex subunit GLTSCR1, but not the closely related BRD7, increase human somatic cell reprogramming efficiency and can replace KLF4 and c-MYC. We find that BRD9 is dispensable for human induced pluripotent stem cells under primed but not under naivStem cell reportsBRD9-containing non-canonical BAF complex maintains somatic cell transcriptome and acts as a barrier to human reprogramming.PMC976857820522MR/V010182/1NAF004\1004Kelekci SCribbs APQi JCan HDunford JESevinc KSevinc GGOppermann UPhilpott MCavga ADSigua LHYıldız ABAta DOnder TTYılmaz AAyar ESArabacı DHfalseBRD9-containing non-canonical BAF complex maintains somatic cell transcriptome and acts as a barrier to human reprogramming.Epigenetic reprogramming to pluripotency requires extensive remodeling of chromatin landscapes to silence existing cell-type-specific genes and activate pluripotency genes. ATP-dependent chromatin remodeling complexes are important regulators of chromatin structure and gene expression; however, the role of recently identified Bromodomain-containing protein 9 (BRD9) and the associated non-canonical BRG1-associated factors (ncBAF) complex in reprogramming remains unknown. Here, we show that genetic or chemical inhibition of BRD9, as well as ncBAF complex subunit GLTSCR1, but not the closely related BRD7, increase human somatic cell reprogramming efficiency and can replace KLF4 and c-MYC. We find that BRD9 is dispensable for human induced pluripotent stem cells under primed but not under naiv2022-01-01T00:00:00Z2022 Dec2026-05-28T21:31:07.503Z2024-10-18T13:12:47.661ZS-EPMC97685783633263110.1016/j.stemcr.2022.10.005