{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["28"],"submitter":["Cardoso-Arenas S"],"pubmed_abstract":["<h4>Background</h4>Scorpion neurotoxins such as those that modify the mammalian voltage-gated sodium ion channels (Nav) are the main responsible for scorpion envenomation. Their neutralization is crucial in the production of antivenoms against scorpion stings.<h4>Methods</h4>In the present study, two <i>in silico</i> designed genes - one that codes for a native neurotoxin from the venom of the Anatolian scorpion <i>Androctonus crassicauda,</i> named Acra 4 - and another non-native toxin - named consensus scorpion toxin (SccTx) obtained from the alignment of the primary structures of the most toxic neurotoxins from the Middle Eastern and North African scorpions - were recombinantly expressed in <i>E. coli</i> Origami.<h4>Results</h4>Following bacterial expression, the two expressed neurotox"],"journal":["The journal of venomous animals and toxins including tropical diseases"],"pagination":["e20220026"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9769139"],"repository":["biostudies-literature"],"pubmed_title":["Recombinant expression and antigenicity of two peptide families of neurotoxins from <i>Androctonus</i> sp."],"pmcid":["PMC9769139"],"pubmed_authors":["Zamudio F","Clement H","Caliskan F","Corrales-Garcia LL","Corzo G","Cardoso-Arenas S","Arenas I","Olvera F"],"additional_accession":[]},"is_claimable":false,"name":"Recombinant expression and antigenicity of two peptide families of neurotoxins from <i>Androctonus</i> sp.","description":"<h4>Background</h4>Scorpion neurotoxins such as those that modify the mammalian voltage-gated sodium ion channels (Nav) are the main responsible for scorpion envenomation. Their neutralization is crucial in the production of antivenoms against scorpion stings.<h4>Methods</h4>In the present study, two <i>in silico</i> designed genes - one that codes for a native neurotoxin from the venom of the Anatolian scorpion <i>Androctonus crassicauda,</i> named Acra 4 - and another non-native toxin - named consensus scorpion toxin (SccTx) obtained from the alignment of the primary structures of the most toxic neurotoxins from the Middle Eastern and North African scorpions - were recombinantly expressed in <i>E. coli</i> Origami.<h4>Results</h4>Following bacterial expression, the two expressed neurotox","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-28T04:38:54.49Z","creation":"2024-11-20T17:36:42.212Z"},"accession":"S-EPMC9769139","cross_references":{"pubmed":["36578820"],"doi":["10.1590/1678-9199-JVATITD-2022-0026"]}}