biostudies-literatureUnknown28Cardoso-Arenas S<h4>Background</h4>Scorpion neurotoxins such as those that modify the mammalian voltage-gated sodium ion channels (Nav) are the main responsible for scorpion envenomation. Their neutralization is crucial in the production of antivenoms against scorpion stings.<h4>Methods</h4>In the present study, two <i>in silico</i> designed genes - one that codes for a native neurotoxin from the venom of the Anatolian scorpion <i>Androctonus crassicauda,</i> named Acra 4 - and another non-native toxin - named consensus scorpion toxin (SccTx) obtained from the alignment of the primary structures of the most toxic neurotoxins from the Middle Eastern and North African scorpions - were recombinantly expressed in <i>E. coli</i> Origami.<h4>Results</h4>Following bacterial expression, the two expressed neurotoxThe journal of venomous animals and toxins including tropical diseasese20220026https://www.ebi.ac.uk/biostudies/studies/S-EPMC9769139biostudies-literatureRecombinant expression and antigenicity of two peptide families of neurotoxins from <i>Androctonus</i> sp.PMC9769139Zamudio FClement HCaliskan FCorrales-Garcia LLCorzo GCardoso-Arenas SArenas IOlvera FfalseRecombinant expression and antigenicity of two peptide families of neurotoxins from <i>Androctonus</i> sp.<h4>Background</h4>Scorpion neurotoxins such as those that modify the mammalian voltage-gated sodium ion channels (Nav) are the main responsible for scorpion envenomation. Their neutralization is crucial in the production of antivenoms against scorpion stings.<h4>Methods</h4>In the present study, two <i>in silico</i> designed genes - one that codes for a native neurotoxin from the venom of the Anatolian scorpion <i>Androctonus crassicauda,</i> named Acra 4 - and another non-native toxin - named consensus scorpion toxin (SccTx) obtained from the alignment of the primary structures of the most toxic neurotoxins from the Middle Eastern and North African scorpions - were recombinantly expressed in <i>E. coli</i> Origami.<h4>Results</h4>Following bacterial expression, the two expressed neurotox2022-01-01T00:00:00Z20222026-05-28T04:38:54.49Z2024-11-20T17:36:42.212ZS-EPMC97691393657882010.1590/1678-9199-JVATITD-2022-0026