biostudies-literatureQiu MZhanjiang Competitive Allocation Project of Science and Technology Development Special FundGuangdong Provincial Special Fund for Modern Agricultural Industry Technology Innovation TeamZhanjing Competitive Allocation Project of Science and Technology Development Special FundGuangdong Provincial Special Foundation for Modern Agriculture Industry Technology Innovation TeamGuangdong Provincial Key Research and Development ProgramNational Natural Science Foundation of ChinaCharacteristic Innovation Project of Colleges and Universities in Guangdong Provincee0368222https://www.ebi.ac.uk/biostudies/studies/S-EPMC9769839biostudies-literatureUnknown10(6)Fusarium oxysporum is ubiquitous and can easily contaminate food during processing and storage, potentially producing T-2 toxin, which can pose a severe health risk to public health. Previous research on the presence of T-2 has focused on starch-rich foods, while protein- and amino acid-rich foods have received relatively little attention. In this study, the effects of amino acids on the growth of F. oxysporum and its T-2 production were investigated by gene deletion and complementation experiments. The results showed that amino acids, including aspartic acid, methionine, isoleucine, serine, phenylalanine, and cysteine, significantly inhibited the growth of F. oxysporum, while promoting T-2 synthesis, with cysteine having the most pronounced effect. The target of rapamycin complex 1 (TORC1Microbiology spectrumCysteine Inhibits the Growth of Fusarium oxysporum and Promotes T-2 Toxin Synthesis through the Gtr/Tap42 Pathway.PMC97698392009151345415772021KJ149321722152020KQNCX0252021B020260001318718982020A030092021KJ151Sun LFang ZDeng QHuang XQiu MWang YZhao JDeng YfalseCysteine Inhibits the Growth of Fusarium oxysporum and Promotes T-2 Toxin Synthesis through the Gtr/Tap42 Pathway.Fusarium oxysporum is ubiquitous and can easily contaminate food during processing and storage, potentially producing T-2 toxin, which can pose a severe health risk to public health. Previous research on the presence of T-2 has focused on starch-rich foods, while protein- and amino acid-rich foods have received relatively little attention. In this study, the effects of amino acids on the growth of F. oxysporum and its T-2 production were investigated by gene deletion and complementation experiments. The results showed that amino acids, including aspartic acid, methionine, isoleucine, serine, phenylalanine, and cysteine, significantly inhibited the growth of F. oxysporum, while promoting T-2 synthesis, with cysteine having the most pronounced effect. The target of rapamycin complex 1 (TORC12022-01-01T00:00:00Z2022 Dec2026-06-12T09:58:40.275Z2024-11-19T17:29:07.588ZS-EPMC97698393631498210.1128/spectrum.03682-22