biostudies-literaturePapazoglou ANational Institute of Arthritis and Musculoskeletal and Skin DiseasesNIAID NIH HHSBurroughs Wellcome FundNHLBI NIH HHSNIAMS NIH HHS2003-2014https://www.ebi.ac.uk/biostudies/studies/S-EPMC9771864biostudies-literatureUnknown74(12)<h4>Objective</h4>Systemic sclerosis-associated interstitial lung disease (SSc-ILD) is the leading cause of death in patients with SSc with unclear pathogenesis and limited treatment options. Evidence strongly supports an important role for profibrotic secreted phosphoprotein 1 (SPP1)-expressing macrophages in SSc-ILD. This study was undertaken to define the transcriptome and chromatin structural changes of SPP1 SSc-ILD macrophages in order to better understand their role in promoting fibrosis and to identify transcription factors associated with open chromatin driving their altered phenotype.<h4>Methods</h4>We performed single-cell RNA sequencing (scRNA-Seq) on 11 explanted SSc-ILD and healthy control lung samples, as well as single-cell assay for transposase-accessible chromatin sequenciArthritis & rheumatology (Hoboken, N.J.)Epigenetic Regulation of Profibrotic Macrophages in Systemic Sclerosis-Associated Interstitial Lung Disease.PMC9771864The University of Pittsburgh holds a Physician‐SciP50 AR0607802P50AR060780T32 AI089443R01 HL123766Morse CRojas MPapazoglou ATabib TSembrat JHuang MLafyatis RLafyatis ABulik MValenzi EfalseEpigenetic Regulation of Profibrotic Macrophages in Systemic Sclerosis-Associated Interstitial Lung Disease.<h4>Objective</h4>Systemic sclerosis-associated interstitial lung disease (SSc-ILD) is the leading cause of death in patients with SSc with unclear pathogenesis and limited treatment options. Evidence strongly supports an important role for profibrotic secreted phosphoprotein 1 (SPP1)-expressing macrophages in SSc-ILD. This study was undertaken to define the transcriptome and chromatin structural changes of SPP1 SSc-ILD macrophages in order to better understand their role in promoting fibrosis and to identify transcription factors associated with open chromatin driving their altered phenotype.<h4>Methods</h4>We performed single-cell RNA sequencing (scRNA-Seq) on 11 explanted SSc-ILD and healthy control lung samples, as well as single-cell assay for transposase-accessible chromatin sequenci2022-01-01T00:00:00Z2022 Dec2025-04-22T13:16:21.21Z2025-04-06T00:33:35.467ZS-EPMC97718643584980310.1002/art.42286