biostudies-literatureMutukuri TTNIAID NIH HHS40-50https://www.ebi.ac.uk/biostudies/studies/S-EPMC9772299biostudies-literatureUnknown112(1)This study examined physical stability of spray freeze dried (SFD) bovine serum albumin (BSA) solids produced using the radio frequency (RF)-assisted drying technique. BSA formulations were prepared with varying concentrations of trehalose and mannitol, using an excipient-free formulation as control. These formulations were produced using either traditional ultrasonic spray freeze drying (SFD) or RF-assisted ultrasonic spray freeze drying (RFSFD). The dried formulations were then characterized using Karl Fischer moisture content measurement, powder X-ray diffraction (PXRD), size exclusion chromatography (SEC), and solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS). Moisture content did not have a good correlation with the physical stability of the formulations measurJournal of pharmaceutical sciencesRadio Frequency - Assisted Ultrasonic Spray Freeze Drying for Pharmaceutical Protein Solids.PMC9772299R01 AI146160Strongrich ADPeroulis DZhou QTMutukuri TTDarwish AAlexeenko AfalseRadio Frequency - Assisted Ultrasonic Spray Freeze Drying for Pharmaceutical Protein Solids.This study examined physical stability of spray freeze dried (SFD) bovine serum albumin (BSA) solids produced using the radio frequency (RF)-assisted drying technique. BSA formulations were prepared with varying concentrations of trehalose and mannitol, using an excipient-free formulation as control. These formulations were produced using either traditional ultrasonic spray freeze drying (SFD) or RF-assisted ultrasonic spray freeze drying (RFSFD). The dried formulations were then characterized using Karl Fischer moisture content measurement, powder X-ray diffraction (PXRD), size exclusion chromatography (SEC), and solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS). Moisture content did not have a good correlation with the physical stability of the formulations measur2023-01-01T00:00:00Z2023 Jan2025-04-26T07:52:44.168Z2025-04-06T12:28:06.627ZS-EPMC97722993618187510.1016/j.xphs.2022.09.024