biostudies-literatureGirard TJNCATS NIH HHSNIAID NIH HHSNHLBI NIH HHSNCI NIH HHS629-638https://www.ebi.ac.uk/biostudies/studies/S-EPMC9773443biostudies-literatureUnknown21(3)<h4>Background</h4>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with excessive coagulation, thrombosis, and mortality.<h4>Objective</h4>To provide insight into mechanisms that contribute to excessive coagulation in coronavirus 2019 (COVID-19) disease.<h4>Patients/methods</h4>Blood from COVID-19 patients was investigated for coagulation-related gene expression and functional activities.<h4>Results</h4>Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells from severe COVID-19 patients revealed a 5.2-fold increase in tissue factor (TF [F3 gene]) transcript expression levels (P < .05), the trigger of extrinsic coagulation; a 7.7-fold increase in C1-inhibitor (SERPING1 gene; P < .01) transcript expression levels, an inhibitor of intJournal of thrombosis and haemostasis : JTHPeripheral blood mononuclear cell tissue factor (F3 gene) transcript levels and circulating extracellular vesicles are elevated in severe coronavirus 2019 (COVID-19) disease.PMC9773443P30 CA091842UL1 TR002345R01 HL120728R61 HL141794R37 AI049653Randolph GJDi Paola JAntunes LSteed ALGirard TJEldem IErlich ECZhang NMazer MCruchaga CRemy KESubramanian RAmrute JMfalsePeripheral blood mononuclear cell tissue factor (F3 gene) transcript levels and circulating extracellular vesicles are elevated in severe coronavirus 2019 (COVID-19) disease.<h4>Background</h4>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with excessive coagulation, thrombosis, and mortality.<h4>Objective</h4>To provide insight into mechanisms that contribute to excessive coagulation in coronavirus 2019 (COVID-19) disease.<h4>Patients/methods</h4>Blood from COVID-19 patients was investigated for coagulation-related gene expression and functional activities.<h4>Results</h4>Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells from severe COVID-19 patients revealed a 5.2-fold increase in tissue factor (TF [F3 gene]) transcript expression levels (P < .05), the trigger of extrinsic coagulation; a 7.7-fold increase in C1-inhibitor (SERPING1 gene; P < .01) transcript expression levels, an inhibitor of int2023-01-01T00:00:00Z2023 Mar2026-05-28T19:00:37.262Z2025-02-19T00:14:00.445ZS-EPMC97734433669618010.1016/j.jtha.2022.11.033