biostudies-literatureRettenbacher LAH2020 Marie Skłodowska-Curie Actions268https://www.ebi.ac.uk/biostudies/studies/S-EPMC9773447biostudies-literatureUnknown21(1)<h4>Background</h4>Escherichia coli is of central interest to biotechnological research and a widely used organism for producing proteins at both lab and industrial scales. However, many proteins remain difficult to produce efficiently in E. coli. This is particularly true for proteins that require post translational modifications such as disulfide bonds.<h4>Results</h4>In this study we develop a novel approach for quantitatively investigating the ability of E. coli to produce disulfide bonds in its own proteome. We summarise the existing knowledge of the E. coli disulfide proteome and use this information to investigate the demand on this organism's quantitative oxidative folding apparatus under different growth conditions. Furthermore, we built an ordinary differential equation-based modMicrobial cell factoriesA quantitative interpretation of oxidative protein folding activity in Escherichia coli.PMC9773447813979Rettenbacher LAvon der Haar TfalseA quantitative interpretation of oxidative protein folding activity in Escherichia coli.<h4>Background</h4>Escherichia coli is of central interest to biotechnological research and a widely used organism for producing proteins at both lab and industrial scales. However, many proteins remain difficult to produce efficiently in E. coli. This is particularly true for proteins that require post translational modifications such as disulfide bonds.<h4>Results</h4>In this study we develop a novel approach for quantitatively investigating the ability of E. coli to produce disulfide bonds in its own proteome. We summarise the existing knowledge of the E. coli disulfide proteome and use this information to investigate the demand on this organism's quantitative oxidative folding apparatus under different growth conditions. Furthermore, we built an ordinary differential equation-based mod2022-01-01T00:00:00Z2022 Dec2026-06-22T03:17:11.633Z2025-02-19T01:55:39.118ZS-EPMC97734473655049510.1186/s12934-022-01982-3