biostudies-literatureLi Sthe Special Funds for Construction of Innovative Provinces in Hunan Province141https://www.ebi.ac.uk/biostudies/studies/S-EPMC9773467biostudies-literatureUnknown18(1)<h4>Background</h4>Camellia oleifera (C. oleifera) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic research. The protoplast and transient transformation system plays an important role in biological breeding, plant regeneration and somatic cell fusion. The objective of this present study was to develop a highly efficient protocol for isolating and purifying mesophyll protoplasts and transient transformation of C. oleifera. Several critical factors for mesophyll protoplast isolation from C. oleifera, including starting material (leaf age), pretreatment, enzymatic treatment (type of enzyme, concentration and digestion time), osmotic pressure and purification were optimizPlant methodsIsolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera.PMC97734672021NK1007Xu LYuan DLi SZhao RZhang JMa XYe TGuan RXiao SfalseIsolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera.<h4>Background</h4>Camellia oleifera (C. oleifera) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic research. The protoplast and transient transformation system plays an important role in biological breeding, plant regeneration and somatic cell fusion. The objective of this present study was to develop a highly efficient protocol for isolating and purifying mesophyll protoplasts and transient transformation of C. oleifera. Several critical factors for mesophyll protoplast isolation from C. oleifera, including starting material (leaf age), pretreatment, enzymatic treatment (type of enzyme, concentration and digestion time), osmotic pressure and purification were optimiz2022-01-01T00:00:00Z2022 Dec2025-04-18T16:33:19.585Z2025-04-07T03:47:08.799ZS-EPMC97734673655055810.1186/s13007-022-00972-1