biostudies-literatureUmbaugh DSMidwest Stem Cell CenterNational Institute of General Medicine Liver Disease COBRENIDDK NIH HHSNational Institute of Diabetes and Digestive and Kidney DiseasesNIGMS NIH HHSMidwest Biomedical Accelerator Consortium, an NIH Research Evaluation and Commercialization Hub3315-3329https://www.ebi.ac.uk/biostudies/studies/S-EPMC9773902biostudies-literatureUnknown96(12)The persistence of hepatotoxicity induced by N-acetyl-para-aminophenol (Acetaminophen or Paracetamol, abbreviated as APAP) as the most common cause of acute liver failure in the United States, despite the availability of N-acetylcysteine, illustrates the clinical relevance of additional therapeutic approaches. While human mesenchymal stem cells (MSCs) have shown protection in mouse models of liver injury, the MSCs used are generally not cleared for human use and it is unclear whether these effects are due to xenotransplantation. Here we evaluated GMP manufactured clinical grade human Wharton's Jelly mesenchymal stem cells (WJMSCs), which are currently being investigated in human clinical trials, in a mouse model of APAP hepatotoxicity in comparison to human dermal fibroblasts (HDFs) to addArchives of toxicologyHuman Wharton's Jelly-derived mesenchymal stem cells prevent acetaminophen-induced liver injury in a mouse model unlike human dermal fibroblasts.PMC9773902P20 GM103549R01 DK102142R01 DK098414P30 GM118247DK125465R01 DK125465Duan LWoolbright BSoder RPRamachandran AUmbaugh DSNguyen NTRobarts DRJaeschke HAdelusi OAbhyankar SDawn BApte UfalseHuman Wharton's Jelly-derived mesenchymal stem cells prevent acetaminophen-induced liver injury in a mouse model unlike human dermal fibroblasts.The persistence of hepatotoxicity induced by N-acetyl-para-aminophenol (Acetaminophen or Paracetamol, abbreviated as APAP) as the most common cause of acute liver failure in the United States, despite the availability of N-acetylcysteine, illustrates the clinical relevance of additional therapeutic approaches. While human mesenchymal stem cells (MSCs) have shown protection in mouse models of liver injury, the MSCs used are generally not cleared for human use and it is unclear whether these effects are due to xenotransplantation. Here we evaluated GMP manufactured clinical grade human Wharton's Jelly mesenchymal stem cells (WJMSCs), which are currently being investigated in human clinical trials, in a mouse model of APAP hepatotoxicity in comparison to human dermal fibroblasts (HDFs) to add2022-01-01T00:00:00Z2022 Dec2025-05-29T21:47:02.503Z2025-05-29T21:47:02.503ZS-EPMC97739023605788610.1007/s00204-022-03372-5