biostudies-literatureUnknown10(12)Shakhov ASActin cytoskeleton is an essential component of living cells and plays a decisive role in many cellular processes. In mammals, β- and γ-actin are cytoplasmic actin isoforms in non-muscle cells. Despite minor differences in the amino acid sequence, β- and γ-actin localize in different cell structures and perform different functions. While cytoplasmic β-actin is involved in many intracellular processes including cell contraction, γ-actin is responsible for cell mobility and promotes tumor transformation. Numerous studies demonstrate that β- and γ-actin are spatially separated in the cytoplasm of fibroblasts and epithelial cells; this separation is functionally determined. The spatial location of β/γ-actin in endothelial cells is still a subject for discussion. Using super-resolution microscoBiomedicines3194https://www.ebi.ac.uk/biostudies/studies/S-EPMC9775052biostudies-literatureColocalization Analysis of Cytoplasmic Actin Isoforms Distribution in Endothelial Cells.PMC9775052Churkina ASShakhov ASKireev IIKovaleva PAAlieva IBfalseColocalization Analysis of Cytoplasmic Actin Isoforms Distribution in Endothelial Cells.Actin cytoskeleton is an essential component of living cells and plays a decisive role in many cellular processes. In mammals, β- and γ-actin are cytoplasmic actin isoforms in non-muscle cells. Despite minor differences in the amino acid sequence, β- and γ-actin localize in different cell structures and perform different functions. While cytoplasmic β-actin is involved in many intracellular processes including cell contraction, γ-actin is responsible for cell mobility and promotes tumor transformation. Numerous studies demonstrate that β- and γ-actin are spatially separated in the cytoplasm of fibroblasts and epithelial cells; this separation is functionally determined. The spatial location of β/γ-actin in endothelial cells is still a subject for discussion. Using super-resolution microsco2022-01-01T00:00:00Z2022 Dec2025-04-07T04:47:01.879Z2025-04-07T04:47:01.879ZS-EPMC97750523655195010.3390/biomedicines10123194