{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(12)"],"submitter":["de Souza Freire L"],"pubmed_abstract":["The COVID-19 pandemic has emphasized the importance and urgent need for rapid and accurate diagnostic tests for detecting and screening this infection. Our proposal was to develop a biosensor based on an ELISA immunoassay for monitoring antibodies against SARS-CoV-2 in human serum samples. The nucleocapsid protein (N protein) from SARS-CoV-2 was employed as a specific receptor for the detection of SARS-CoV-2 nucleocapsid immunoglobulin G. N protein was immobilized on the surface of a screen-printed carbon electrode (SPCE) modified with carboxylated graphene (CG). The percentage of IgG-SARS-CoV-2 nucleocapsid present was quantified using a secondary antibody labeled with horseradish peroxidase (HRP) (anti-IgG-HRP) catalyzed using 3,3',5,5'-tetramethylbenzidine (TMB) mediator by chronoamperometry. A linear response was obtained in the range of 1:1000-1:200 <i>v/v</i> in phosphate buffer solution (PBS), and the detection limit calculated was 1:4947 <i>v</i>/<i>v</i>. The chronoamperometric method showed electrical signals directly proportional to antibody concentrations due to antigen-antibody (Ag-Ab) specific and stable binding reaction."],"journal":["Biosensors"],"pagination":["1161"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9775996"],"repository":["biostudies-literature"],"pubmed_title":["An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination."],"pmcid":["PMC9775996"],"pubmed_authors":["Ruzo CM","de Souza Freire L","Salgado BB","Lalwani JDB","Talu S","Matos R","Romaguera-Barcelay Y","Fonseca Filho H","Brito WR","Lalwani P","Sales MGF","Cordeiro I","Tavares APM","Astolfi-Filho S","Gandarilla AMD"],"additional_accession":[]},"is_claimable":false,"name":"An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination.","description":"The COVID-19 pandemic has emphasized the importance and urgent need for rapid and accurate diagnostic tests for detecting and screening this infection. Our proposal was to develop a biosensor based on an ELISA immunoassay for monitoring antibodies against SARS-CoV-2 in human serum samples. The nucleocapsid protein (N protein) from SARS-CoV-2 was employed as a specific receptor for the detection of SARS-CoV-2 nucleocapsid immunoglobulin G. N protein was immobilized on the surface of a screen-printed carbon electrode (SPCE) modified with carboxylated graphene (CG). The percentage of IgG-SARS-CoV-2 nucleocapsid present was quantified using a secondary antibody labeled with horseradish peroxidase (HRP) (anti-IgG-HRP) catalyzed using 3,3',5,5'-tetramethylbenzidine (TMB) mediator by chronoamperometry. A linear response was obtained in the range of 1:1000-1:200 <i>v/v</i> in phosphate buffer solution (PBS), and the detection limit calculated was 1:4947 <i>v</i>/<i>v</i>. The chronoamperometric method showed electrical signals directly proportional to antibody concentrations due to antigen-antibody (Ag-Ab) specific and stable binding reaction.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-18T16:00:46.46Z","creation":"2025-04-07T02:55:35.482Z"},"accession":"S-EPMC9775996","cross_references":{"pubmed":["36551128"],"doi":["10.3390/bios12121161"]}}