biostudies-literatureBicka MNational Science CentreEuropean Social Fund Project NCBiR4048https://www.ebi.ac.uk/biostudies/studies/S-EPMC9776847biostudies-literatureUnknown11(24)Motile cilia and eukaryotic flagella are specific cell protrusions that are conserved from protists to humans. They are supported by a skeleton composed of uniquely organized microtubules-nine peripheral doublets and two central singlets (9 × 2 + 2). Microtubules also serve as docking sites for periodically distributed multiprotein ciliary complexes. Radial spokes, the T-shaped ciliary complexes, repeat along the outer doublets as triplets and transduce the regulatory signals from the cilium center to the outer doublet-docked dynein arms. Using the genetic, proteomic, and microscopic approaches, we have shown that lack of <i>Tetrahymena</i> Cfap91 protein affects stable docking/positioning of the radial spoke RS3 and the base of RS2, and adjacent inner dynein arms, possibly due to the abilCellsCfap91-Dependent Stability of the RS2 and RS3 Base Proteins and Adjacent Inner Dynein Arms in <i>Tetrahymena</i> Cilia.PMC9776847PBS3/A8/36/2015OPUS15 2018/29/B/NZ3/02443OPUS13 2017/25/B/NZ3/01609Urbanska PKonopka ABicka MOsinka ABulska EWloga DJoachimiak EfalseCfap91-Dependent Stability of the RS2 and RS3 Base Proteins and Adjacent Inner Dynein Arms in <i>Tetrahymena</i> Cilia.Motile cilia and eukaryotic flagella are specific cell protrusions that are conserved from protists to humans. They are supported by a skeleton composed of uniquely organized microtubules-nine peripheral doublets and two central singlets (9 × 2 + 2). Microtubules also serve as docking sites for periodically distributed multiprotein ciliary complexes. Radial spokes, the T-shaped ciliary complexes, repeat along the outer doublets as triplets and transduce the regulatory signals from the cilium center to the outer doublet-docked dynein arms. Using the genetic, proteomic, and microscopic approaches, we have shown that lack of <i>Tetrahymena</i> Cfap91 protein affects stable docking/positioning of the radial spoke RS3 and the base of RS2, and adjacent inner dynein arms, possibly due to the abil2022-01-01T00:00:00Z2022 Dec2026-04-08T18:26:02.781Z2025-04-04T14:52:02.209ZS-EPMC97768473655281110.3390/cells11244048