biostudies-literatureZhu LChina Agriculture Research SystemSAAS Program for Excellent Research Team 20224133https://www.ebi.ac.uk/biostudies/studies/S-EPMC9776975biostudies-literatureUnknown11(24)<i>Lactobacillus</i> spp., as probiotics, have shown efficacy in alleviating nonalcoholic fatty liver disease (NAFLD). Here, we screened a new probiotic strain, <i>Lactobacillus salivarius</i> SNK-6 (<i>L. salivarius</i> SNK-6), which was isolated from the ileum of healthy Xinyang black-feather laying hens in China. We investigated the beneficial activity of <i>L. salivarius</i> SNK-6 in a NAFLD model in laying hens and found that <i>L. salivarius</i> SNK-6 inhibited liver fat deposition and decreased serum triglyceride levels and activity of aspartate transaminase and alanine transaminase. MBOAT2 (membrane-bound O-acyltransferase domain containing 2) was directly targeted by miR-130a-5p, which was downregulated in the liver of NAFLD laying hens but reversed after <i>L. salivarius</i> SNK-Cells<i>Lactobacillus salivarius</i> SNK-6 Regulates Liver Lipid Metabolism Partly via the miR-130a-5p/MBOAT2 Pathway in a NAFLD Model of Laying Hens.PMC9776975B-021CARS-40-K03Yang YHe DHuang JZhu LLiao RWang HXiao CYan HYang Cfalse<i>Lactobacillus salivarius</i> SNK-6 Regulates Liver Lipid Metabolism Partly via the miR-130a-5p/MBOAT2 Pathway in a NAFLD Model of Laying Hens.<i>Lactobacillus</i> spp., as probiotics, have shown efficacy in alleviating nonalcoholic fatty liver disease (NAFLD). Here, we screened a new probiotic strain, <i>Lactobacillus salivarius</i> SNK-6 (<i>L. salivarius</i> SNK-6), which was isolated from the ileum of healthy Xinyang black-feather laying hens in China. We investigated the beneficial activity of <i>L. salivarius</i> SNK-6 in a NAFLD model in laying hens and found that <i>L. salivarius</i> SNK-6 inhibited liver fat deposition and decreased serum triglyceride levels and activity of aspartate transaminase and alanine transaminase. MBOAT2 (membrane-bound O-acyltransferase domain containing 2) was directly targeted by miR-130a-5p, which was downregulated in the liver of NAFLD laying hens but reversed after <i>L. salivarius</i> SNK-2022-01-01T00:00:00Z2022 Dec2026-04-08T18:27:26.206Z2025-04-05T08:47:07.349ZS-EPMC97769753655289610.3390/cells11244133