{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao L"],"funding":["Inner Mongolia Autonomous Region Science and Technology Plan Project","Inner Mongolia Autonomous Region Natural Science","National Natural Science Foundation of China"],"pagination":["2296"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9777697"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(12)"],"pubmed_abstract":["β-amylase (BAM) plays an important role in plant development and response to abiotic stresses. In this study, 5 <i>DoBAM</i> members were identified in yam (<i>Dioscorea opposita</i> Thunb.). A novel β-amylase gene <i>BAM1</i>, (named <i>DoBAM1</i>), was isolated from yam varieties Bikeqi and Dahechangyu. The open reading frame (ORF) of <i>DoBAM1</i> is 2806 bp and encodes 543 amino acids. Subcellular localization analysis indicates that DoBAM1 localizes to the cell membrane and cytoplasm. In the yam variety Dahechangyu, the starch content, β-amylase activity, and expression of <i>DoBAM1</i> were characterized and found to all be higher than in Bikeqi. <i>DoBAM1</i> overexpression in tobacco is shown to promote the accumulation of soluble sugar and chlorophyll content and to increase the a"],"journal":["Genes"],"pubmed_title":["Overexpression of <i>DoBAM1</i> from Yam (<i>Dioscorea opposita</i> Thunb.) Enhances Cold Tolerance in Transgenic Tobacco."],"pmcid":["PMC9777697"],"funding_grant_id":["2022MS03052","2020GG0044","31860558"],"pubmed_authors":["Zhao L","Xing L","Shao Y","Zhang Y","Huo X","Ge M"],"additional_accession":[]},"is_claimable":false,"name":"Overexpression of <i>DoBAM1</i> from Yam (<i>Dioscorea opposita</i> Thunb.) Enhances Cold Tolerance in Transgenic Tobacco.","description":"β-amylase (BAM) plays an important role in plant development and response to abiotic stresses. In this study, 5 <i>DoBAM</i> members were identified in yam (<i>Dioscorea opposita</i> Thunb.). A novel β-amylase gene <i>BAM1</i>, (named <i>DoBAM1</i>), was isolated from yam varieties Bikeqi and Dahechangyu. The open reading frame (ORF) of <i>DoBAM1</i> is 2806 bp and encodes 543 amino acids. Subcellular localization analysis indicates that DoBAM1 localizes to the cell membrane and cytoplasm. In the yam variety Dahechangyu, the starch content, β-amylase activity, and expression of <i>DoBAM1</i> were characterized and found to all be higher than in Bikeqi. <i>DoBAM1</i> overexpression in tobacco is shown to promote the accumulation of soluble sugar and chlorophyll content and to increase the a","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-04-08T12:10:00.467Z","creation":"2024-11-21T06:48:37.602Z"},"accession":"S-EPMC9777697","cross_references":{"pubmed":["36553563"],"doi":["10.3390/genes13122296"]}}