biostudies-literatureZhao LInner Mongolia Autonomous Region Science and Technology Plan ProjectInner Mongolia Autonomous Region Natural ScienceNational Natural Science Foundation of China2296https://www.ebi.ac.uk/biostudies/studies/S-EPMC9777697biostudies-literatureUnknown13(12)β-amylase (BAM) plays an important role in plant development and response to abiotic stresses. In this study, 5 <i>DoBAM</i> members were identified in yam (<i>Dioscorea opposita</i> Thunb.). A novel β-amylase gene <i>BAM1</i>, (named <i>DoBAM1</i>), was isolated from yam varieties Bikeqi and Dahechangyu. The open reading frame (ORF) of <i>DoBAM1</i> is 2806 bp and encodes 543 amino acids. Subcellular localization analysis indicates that DoBAM1 localizes to the cell membrane and cytoplasm. In the yam variety Dahechangyu, the starch content, β-amylase activity, and expression of <i>DoBAM1</i> were characterized and found to all be higher than in Bikeqi. <i>DoBAM1</i> overexpression in tobacco is shown to promote the accumulation of soluble sugar and chlorophyll content and to increase the aGenesOverexpression of <i>DoBAM1</i> from Yam (<i>Dioscorea opposita</i> Thunb.) Enhances Cold Tolerance in Transgenic Tobacco.PMC97776972022MS030522020GG004431860558Zhao LXing LShao YZhang YHuo XGe MfalseOverexpression of <i>DoBAM1</i> from Yam (<i>Dioscorea opposita</i> Thunb.) Enhances Cold Tolerance in Transgenic Tobacco.β-amylase (BAM) plays an important role in plant development and response to abiotic stresses. In this study, 5 <i>DoBAM</i> members were identified in yam (<i>Dioscorea opposita</i> Thunb.). A novel β-amylase gene <i>BAM1</i>, (named <i>DoBAM1</i>), was isolated from yam varieties Bikeqi and Dahechangyu. The open reading frame (ORF) of <i>DoBAM1</i> is 2806 bp and encodes 543 amino acids. Subcellular localization analysis indicates that DoBAM1 localizes to the cell membrane and cytoplasm. In the yam variety Dahechangyu, the starch content, β-amylase activity, and expression of <i>DoBAM1</i> were characterized and found to all be higher than in Bikeqi. <i>DoBAM1</i> overexpression in tobacco is shown to promote the accumulation of soluble sugar and chlorophyll content and to increase the a2022-01-01T00:00:00Z2022 Dec2026-04-08T12:10:00.467Z2024-11-21T06:48:37.602ZS-EPMC97776973655356310.3390/genes13122296