biostudies-literatureUnknown13(12)Bashir ABacteriocins are gaining immense importance in therapeutics since they show significant antibacterial potential. This study reports the bacteriocin KAE01 from <i>Enterococcus faecium,</i> along with its characterization, molecular modeling, and antibacterial potency, by targeting the matrix protein of <i>Pseudomonas aeruginosa</i>. The bacteriocin was purified by using ammonium sulfate precipitation and fast protein liquid chromatography (FPLC), and its molecular weight was estimated as 55 kDa by means of SDS-PAGE. The bacteriocin was found to show stability in a wide range of pH values (2.0-10.0) and temperatures (100 °C for 1 h and 121 °C for 15 min). Antimicrobial screening of the purified peptide against different strains of <i>P. aeruginosa</i> showed its significant antibacterial potGenes2333https://www.ebi.ac.uk/biostudies/studies/S-EPMC9777700biostudies-literatureMolecular Characterization, Purification, and Mode of Action of <i>Enterocin</i> KAE01 from Lactic Acid Bacteria and Its In Silico Analysis against MDR/ESBL <i>Pseudomonas aeruginosa</i>.PMC9777700Hussain AFarid NHerwig RHassan KAFatima KKhaireabadi MBux KHaider SABashir AMehmood SAli KfalseMolecular Characterization, Purification, and Mode of Action of <i>Enterocin</i> KAE01 from Lactic Acid Bacteria and Its In Silico Analysis against MDR/ESBL <i>Pseudomonas aeruginosa</i>.Bacteriocins are gaining immense importance in therapeutics since they show significant antibacterial potential. This study reports the bacteriocin KAE01 from <i>Enterococcus faecium,</i> along with its characterization, molecular modeling, and antibacterial potency, by targeting the matrix protein of <i>Pseudomonas aeruginosa</i>. The bacteriocin was purified by using ammonium sulfate precipitation and fast protein liquid chromatography (FPLC), and its molecular weight was estimated as 55 kDa by means of SDS-PAGE. The bacteriocin was found to show stability in a wide range of pH values (2.0-10.0) and temperatures (100 °C for 1 h and 121 °C for 15 min). Antimicrobial screening of the purified peptide against different strains of <i>P. aeruginosa</i> showed its significant antibacterial pot2022-01-01T00:00:00Z2022 Dec2025-04-22T21:37:14.708Z2024-11-12T21:18:52.407ZS-EPMC97777003655359910.3390/genes13122333