{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jiang S"],"funding":["Chinese Agriculture Research System of MOF and MARA of China"],"pagination":["15778"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9778975"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(24)"],"pubmed_abstract":["Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor that binds to the CCAAT <i>cis</i>-element in the promoters of target genes and plays critical roles in plant growth, development, and stress responses. In the present study, we aimed to re-characterize the <i>ClNF-Y</i> family in watermelon, examine the assembly of ClNF-Y complexes, and explore their possible involvement in disease resistance. A total of 25 <i>ClNF-Y</i> genes (7 <i>ClNF-YAs</i>, 10 <i>ClNF-YBs</i>, and 8 <i>ClNF-YCs</i>) were identified in the watermelon genome. The <i>ClNF-Y</i> family was comprehensively characterized in terms of gene and protein structures, phylogenetic relationships, and evolution events. Different types of <i>cis</i>-elements responsible for plant growth and development, phytohormones, and/or stress responses were identified in the promoters of the <i>ClNF-Y</i> genes. ClNF-YAs and ClNF-YCs were mainly localized in the nucleus, while most of the ClNF-YBs were localized in the cytoplasm of cells. ClNF-YB5, -YB6, -YB7, -YB8, -YB9, and -YB10 interacted with ClNF-YC2, -YC3, -YC4, -YC5, -YC6, -YC7, and -YC8, while ClNF-YB1 and -YB3 interacted with ClNF-YC1. A total of 37 putative ClNF-Y complexes were identified, e.g., ClNF-YA1, -YA2, -YA3, and -YA7 assembled into 13, 8, 8, and 8 ClNF-Y complexes with different ClNF-YB/-YC heterodimers. Most of the <i>ClNF-Y</i> genes responded with distinct expression patterns to defense hormones such as salicylic acid, methyl jasmonate, abscisic acid, and ethylene precursor 1-aminocyclopropane-1-carboxylate, and to infection by the vascular infecting fungus <i>Fusarium oxysporum</i> f. sp. <i>niveum</i>. Overexpression of <i>ClNF-YB1</i>, <i>-YB8</i>, <i>-YB9</i>, <i>ClNF-YC2</i>, and <i>-YC7</i> in transgenic Arabidopsis resulted in an earlier flowering phenotype. Overexpression of <i>ClNF-YB8</i> in Arabidopsis led to enhanced resistance while overexpression of <i>ClNF-YA2</i> and <i>-YC2</i> resulted in decreased resistance against <i>Botrytis cinerea</i>. Similarly, overexpression of <i>ClNF-YA3</i>, <i>-YB1</i>, and <i>-YC4</i> strengthened resistance while overexpression of <i>ClNF-YA2</i> and <i>-YB8</i> attenuated resistance against <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000. The re-characterization of the <i>ClNF-Y</i> family provides a basis from which to investigate the biological functions of <i>ClNF-Y</i> genes in respect of growth, development, and stress response in watermelon, and the identification of the functions of some <i>ClNF-Y</i> genes in disease resistance enables further exploration of the molecular mechanism of <i>ClNF-Ys</i> in the regulation of watermelon immunity against diverse pathogens."],"journal":["International journal of molecular sciences"],"pubmed_title":["The NF-Y Transcription Factor Family in Watermelon: Re-Characterization, Assembly of ClNF-Y Complexes, Hormone- and Pathogen-Inducible Expression and Putative Functions in Disease Resistance."],"pmcid":["PMC9778975"],"funding_grant_id":["CARS-25"],"pubmed_authors":["Wen Y","Li D","Liang J","Jiang S","Song F","Wang H"],"additional_accession":[]},"is_claimable":false,"name":"The NF-Y Transcription Factor Family in Watermelon: Re-Characterization, Assembly of ClNF-Y Complexes, Hormone- and Pathogen-Inducible Expression and Putative Functions in Disease Resistance.","description":"Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor that binds to the CCAAT <i>cis</i>-element in the promoters of target genes and plays critical roles in plant growth, development, and stress responses. In the present study, we aimed to re-characterize the <i>ClNF-Y</i> family in watermelon, examine the assembly of ClNF-Y complexes, and explore their possible involvement in disease resistance. A total of 25 <i>ClNF-Y</i> genes (7 <i>ClNF-YAs</i>, 10 <i>ClNF-YBs</i>, and 8 <i>ClNF-YCs</i>) were identified in the watermelon genome. The <i>ClNF-Y</i> family was comprehensively characterized in terms of gene and protein structures, phylogenetic relationships, and evolution events. Different types of <i>cis</i>-elements responsible for plant growth and development, phytohormones, and/or stress responses were identified in the promoters of the <i>ClNF-Y</i> genes. ClNF-YAs and ClNF-YCs were mainly localized in the nucleus, while most of the ClNF-YBs were localized in the cytoplasm of cells. ClNF-YB5, -YB6, -YB7, -YB8, -YB9, and -YB10 interacted with ClNF-YC2, -YC3, -YC4, -YC5, -YC6, -YC7, and -YC8, while ClNF-YB1 and -YB3 interacted with ClNF-YC1. A total of 37 putative ClNF-Y complexes were identified, e.g., ClNF-YA1, -YA2, -YA3, and -YA7 assembled into 13, 8, 8, and 8 ClNF-Y complexes with different ClNF-YB/-YC heterodimers. Most of the <i>ClNF-Y</i> genes responded with distinct expression patterns to defense hormones such as salicylic acid, methyl jasmonate, abscisic acid, and ethylene precursor 1-aminocyclopropane-1-carboxylate, and to infection by the vascular infecting fungus <i>Fusarium oxysporum</i> f. sp. <i>niveum</i>. Overexpression of <i>ClNF-YB1</i>, <i>-YB8</i>, <i>-YB9</i>, <i>ClNF-YC2</i>, and <i>-YC7</i> in transgenic Arabidopsis resulted in an earlier flowering phenotype. Overexpression of <i>ClNF-YB8</i> in Arabidopsis led to enhanced resistance while overexpression of <i>ClNF-YA2</i> and <i>-YC2</i> resulted in decreased resistance against <i>Botrytis cinerea</i>. Similarly, overexpression of <i>ClNF-YA3</i>, <i>-YB1</i>, and <i>-YC4</i> strengthened resistance while overexpression of <i>ClNF-YA2</i> and <i>-YB8</i> attenuated resistance against <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000. The re-characterization of the <i>ClNF-Y</i> family provides a basis from which to investigate the biological functions of <i>ClNF-Y</i> genes in respect of growth, development, and stress response in watermelon, and the identification of the functions of some <i>ClNF-Y</i> genes in disease resistance enables further exploration of the molecular mechanism of <i>ClNF-Ys</i> in the regulation of watermelon immunity against diverse pathogens.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-04-08T11:02:24.021Z","creation":"2024-11-08T14:39:20.586Z"},"accession":"S-EPMC9778975","cross_references":{"pubmed":["36555422"],"doi":["10.3390/ijms232415778"]}}