{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Park B"],"funding":["NEI NIH HHS"],"pagination":["15595"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9779010"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(24)"],"pubmed_abstract":["Neovascular or \"wet\" age-related macular degeneration (nAMD) is a leading cause of blindness among older adults. Choroidal neovascularization (CNV) is a major pathological feature of nAMD, in which abnormal new blood vessel growth from the choroid leads to irreversible vision loss. There is a critical need to develop novel therapeutic strategies to address limitations of the current anti-vascular endothelial growth factor biologics. Previously, we identified soluble epoxide hydrolase (sEH) as a possible therapeutic target for CNV through a forward chemical genetic approach. The purpose of this study was to validate sEH as a target by examining retinal expression of sEH protein and mRNA by immunohistochemistry and RNAscope in situ hybridization, respectively, and to assess the efficacy of an adeno-associated virus (AAV) vector designed to knock down the sEH gene, <i>Ephx2</i>, in the murine laser-induced (L-) CNV model. nAMD patient postmortem eye tissue and murine L-CNV showed overexpression of sEH in photoreceptors and retinal pigment epithelial cells. <i>Ephx2</i> knockdown significantly reduced CNV and normalized mRNA expression levels of CNV-related inflammatory markers. Thus, this study further establishes sEH as a promising therapeutic target against CNV associated with nAMD."],"journal":["International journal of molecular sciences"],"pubmed_title":["Decreased Expression of Soluble Epoxide Hydrolase Suppresses Murine Choroidal Neovascularization."],"pmcid":["PMC9779010"],"funding_grant_id":["R01EY031939","R01 EY025641","R01EY025641","R01 EY031939"],"pubmed_authors":["Sishtla KL","Sardar Pasha SPB","Hartman GD","Qi X","Corson TW","Park B","Boulton ME"],"additional_accession":[]},"is_claimable":false,"name":"Decreased Expression of Soluble Epoxide Hydrolase Suppresses Murine Choroidal Neovascularization.","description":"Neovascular or \"wet\" age-related macular degeneration (nAMD) is a leading cause of blindness among older adults. Choroidal neovascularization (CNV) is a major pathological feature of nAMD, in which abnormal new blood vessel growth from the choroid leads to irreversible vision loss. There is a critical need to develop novel therapeutic strategies to address limitations of the current anti-vascular endothelial growth factor biologics. Previously, we identified soluble epoxide hydrolase (sEH) as a possible therapeutic target for CNV through a forward chemical genetic approach. The purpose of this study was to validate sEH as a target by examining retinal expression of sEH protein and mRNA by immunohistochemistry and RNAscope in situ hybridization, respectively, and to assess the efficacy of an adeno-associated virus (AAV) vector designed to knock down the sEH gene, <i>Ephx2</i>, in the murine laser-induced (L-) CNV model. nAMD patient postmortem eye tissue and murine L-CNV showed overexpression of sEH in photoreceptors and retinal pigment epithelial cells. <i>Ephx2</i> knockdown significantly reduced CNV and normalized mRNA expression levels of CNV-related inflammatory markers. Thus, this study further establishes sEH as a promising therapeutic target against CNV associated with nAMD.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-04T08:45:30.751Z","creation":"2025-04-04T08:45:30.751Z"},"accession":"S-EPMC9779010","cross_references":{"pubmed":["36555236"],"doi":["10.3390/ijms232415595"]}}