biostudies-literatureZiesel AIntramural research program, Center for Cancer Research, National Cancer InstituteNational Institutes of Health General MedicinePrivate donation from Eugene and Carol ChengNIGMS NIH HHSe1010407https://www.ebi.ac.uk/biostudies/studies/S-EPMC9779700biostudies-literatureUnknown18(12)During meiosis, recombination between homologous chromosomes (homologs) generates crossovers that promote proper segregation at the first meiotic division. Recombination is initiated by Spo11-catalyzed DNA double strand breaks (DSBs). 5' end resection of the DSBs creates 3' single strand tails that two recombinases, Rad51 and Dmc1, bind to form presynaptic filaments that search for homology, mediate strand invasion and generate displacement loops (D-loops). D-loop processing then forms crossover and non-crossover recombinants. Meiotic recombination occurs in two temporally distinct phases. During Phase 1, Rad51 is inhibited and Dmc1 mediates the interhomolog recombination that promotes homolog synapsis. In Phase 2, Rad51 becomes active and functions with Rad54 to repair residual DSBs, makiPLoS geneticsRad51-mediated interhomolog recombination during budding yeast meiosis is promoted by the meiotic recombination checkpoint and the conserved Pif1 helicase.PMC9779700GM R01 050717; R35 140684GM R01 125800R35 GM140684Ziesel AWeng QCheng EBhattacharya ADutta RLichten MAhuja JSHollingsworth NMBorner GVfalseRad51-mediated interhomolog recombination during budding yeast meiosis is promoted by the meiotic recombination checkpoint and the conserved Pif1 helicase.During meiosis, recombination between homologous chromosomes (homologs) generates crossovers that promote proper segregation at the first meiotic division. Recombination is initiated by Spo11-catalyzed DNA double strand breaks (DSBs). 5' end resection of the DSBs creates 3' single strand tails that two recombinases, Rad51 and Dmc1, bind to form presynaptic filaments that search for homology, mediate strand invasion and generate displacement loops (D-loops). D-loop processing then forms crossover and non-crossover recombinants. Meiotic recombination occurs in two temporally distinct phases. During Phase 1, Rad51 is inhibited and Dmc1 mediates the interhomolog recombination that promotes homolog synapsis. In Phase 2, Rad51 becomes active and functions with Rad54 to repair residual DSBs, maki2022-01-01T00:00:00Z2022 Dec2025-04-04T13:49:08.668Z2025-04-04T13:49:08.668ZS-EPMC97797003650846810.1371/journal.pgen.1010407