biostudies-literatureUnknown27(24)Ali SGAntimicrobial resistance has posed a serious health concern worldwide, which is mainly due to the excessive use of antibiotics. In this study, gold nanoparticles synthesized from the plant <i>Tinospora cordifolia</i> were used against multidrug-resistant <i>Pseudomonas aeruginosa</i>. The active components involved in the reduction and stabilization of gold nanoparticles were revealed by gas chromatography-mass spectrophotometry(GC-MS) of the stem extract of <i>Tinospora cordifolia.</i> Gold nanoparticles (TG-AuNPs) were effective against <i>P. aeruginosa</i> at different concentrations (50,100, and 150 µg/mL). TG-AuNPs effectively reduced the pyocyanin level by 63.1% in PAO1 and by 68.7% in clinical isolates at 150 µg/mL; similarly, swarming and swimming motilities decreased by 53.1% and Molecules (Basel, Switzerland)8685https://www.ebi.ac.uk/biostudies/studies/S-EPMC9781250biostudies-literatureBiosynthesis of Gold Nanoparticles and Its Effect against <i>Pseudomonas aeruginosa</i>.PMC9781250Ahmad AAlshammari MBAli SGAhmad HUmar KJalal MKhan HMfalseBiosynthesis of Gold Nanoparticles and Its Effect against <i>Pseudomonas aeruginosa</i>.Antimicrobial resistance has posed a serious health concern worldwide, which is mainly due to the excessive use of antibiotics. In this study, gold nanoparticles synthesized from the plant <i>Tinospora cordifolia</i> were used against multidrug-resistant <i>Pseudomonas aeruginosa</i>. The active components involved in the reduction and stabilization of gold nanoparticles were revealed by gas chromatography-mass spectrophotometry(GC-MS) of the stem extract of <i>Tinospora cordifolia.</i> Gold nanoparticles (TG-AuNPs) were effective against <i>P. aeruginosa</i> at different concentrations (50,100, and 150 µg/mL). TG-AuNPs effectively reduced the pyocyanin level by 63.1% in PAO1 and by 68.7% in clinical isolates at 150 µg/mL; similarly, swarming and swimming motilities decreased by 53.1% and 2022-01-01T00:00:00Z2022 Dec2025-04-04T07:59:17.888Z2025-04-04T07:59:17.888ZS-EPMC97812503655781810.3390/molecules27248685