biostudies-literatureSchwarze MDeutsche Forschungsgemeinschaft (DFG)European Regional Development Fund (ERDF) and the Free State of SaxonyDeutsche ForschungsgemeinschaftEuropean Union, the European Regional Development Fund (ERDF) and the Free State of SaxonyOpen Access Publishing Fund of Leipzig University1515https://www.ebi.ac.uk/biostudies/studies/S-EPMC9782079biostudies-literatureUnknown11(12)This study investigated the IgG and IgA antibody response against recombinant S1 and receptor binding domains (RBD) of the spike (S-) protein and the membrane (M-) protein using a set of 115 serum samples collected from patients infected with SARS-CoV-2 in Germany before April 2021 using protein and peptide ELISA. As S1- and RBD-proteins expressed in <i>Escherichia coli</i> provided poor sensitivities in ELISA, they were replaced by proteins expressed in HEK cells. The RBD-ELISA provided a sensitivity of 90.6% (N = 85) for samples collected from patients with confirmed SARS-CoV-2 infections more than 14 days after symptom onset or a positive PCR test. In population-based controls, the specificity was 97.9% (N = 94). In contrast, the sensitivities were only 41.2% and 72.6% for M- and N-protPathogens (Basel, Switzerland)Evaluation of S- and M-Proteins Expressed in <i>Escherichia coli</i> and HEK Cells for Serological Detection of Antibodies in Response to SARS-CoV-2 Infections and mRNA-Based Vaccinations.PMC9782079100523073INST 268/387-1Schwarze MScholz MGabert JBorte SMilkovska-Stamenova SGrunewald TBrakel ALakowa NLuo JWolf JKrizsan ALehmann CHoffmann RfalseEvaluation of S- and M-Proteins Expressed in <i>Escherichia coli</i> and HEK Cells for Serological Detection of Antibodies in Response to SARS-CoV-2 Infections and mRNA-Based Vaccinations.This study investigated the IgG and IgA antibody response against recombinant S1 and receptor binding domains (RBD) of the spike (S-) protein and the membrane (M-) protein using a set of 115 serum samples collected from patients infected with SARS-CoV-2 in Germany before April 2021 using protein and peptide ELISA. As S1- and RBD-proteins expressed in <i>Escherichia coli</i> provided poor sensitivities in ELISA, they were replaced by proteins expressed in HEK cells. The RBD-ELISA provided a sensitivity of 90.6% (N = 85) for samples collected from patients with confirmed SARS-CoV-2 infections more than 14 days after symptom onset or a positive PCR test. In population-based controls, the specificity was 97.9% (N = 94). In contrast, the sensitivities were only 41.2% and 72.6% for M- and N-prot2022-01-01T00:00:00Z2022 Dec2026-04-08T10:58:57.835Z2024-11-15T17:28:13.086ZS-EPMC97820793655884910.3390/pathogens11121515