biostudies-literatureKempa JNCRR NIH HHSNIDDK NIH HHSNIAID NIH HHSMurdock Charitable Trust FoundationMSU’s office of the Vice President for Research, Economic Development, and Graduate Education at MSUUSPHSNIH SIG programNational Science Foundation815https://www.ebi.ac.uk/biostudies/studies/S-EPMC9782143biostudies-literatureUnknown14(12)Hypoglycemia may be induced by a variety of physiologic and pathologic stimuli and can result in life-threatening consequences if untreated. However, hypoglycemia may also play a role in the purported health benefits of intermittent fasting and caloric restriction. Previously, we demonstrated that systemic administration of ricin toxin induced fatal hypoglycemia in mice. Here, we examine the metabolic landscape of the hypoglycemic state induced in the liver of mice by two different stimuli: systemic ricin administration and fasting. Each stimulus produced the same decrease in blood glucose and weight loss. The polar metabolome was studied using ¹H NMR, quantifying 59 specific metabolites, and untargeted LC-MS on approximately 5000 features. Results were analyzed by multivariate ToxinsDistinct Metabolic States Are Observed in Hypoglycemia Induced in Mice by Ricin Toxin or by Fasting.PMC9782143AI136758(SP)R01 AI136758S10 RR026659R56 AI127398NSF-MRI:DBI-15320781S10RR138781S10RR026659DK117473AI1273982015066:MNLR01 AI059376NSF-MRI:CHE-2018388R01DK117473R01 DK117473Copie VPeters TMoss CEBothner BO'Shea-Stone GTripet BPincus SHMarcotte TKKempa JEilers BfalseDistinct Metabolic States Are Observed in Hypoglycemia Induced in Mice by Ricin Toxin or by Fasting.Hypoglycemia may be induced by a variety of physiologic and pathologic stimuli and can result in life-threatening consequences if untreated. However, hypoglycemia may also play a role in the purported health benefits of intermittent fasting and caloric restriction. Previously, we demonstrated that systemic administration of ricin toxin induced fatal hypoglycemia in mice. Here, we examine the metabolic landscape of the hypoglycemic state induced in the liver of mice by two different stimuli: systemic ricin administration and fasting. Each stimulus produced the same decrease in blood glucose and weight loss. The polar metabolome was studied using ¹H NMR, quantifying 59 specific metabolites, and untargeted LC-MS on approximately 5000 features. Results were analyzed by multivariate 2022-01-01T00:00:00Z2022 Nov2025-04-04T21:55:22.976Z2025-02-19T01:55:55.452ZS-EPMC97821433654871210.3390/toxins14120815