{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Suzuki N"],"funding":["Moonshot Research and Development Program of the New Energy and Industrial Technology Development Organization, Japan","Japan Science and Technology Agency","New Energy and Industrial Technology Development Organization"],"pagination":["2324"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9782182"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(12)"],"pubmed_abstract":["Microbial degradation of natural rubber and synthetic poly(<i>cis</i>-1,4-isoprene) is expected to become an alternative treatment system for waste from poly(<i>cis</i>-1,4-isoprene) products including scrap tires. <i>Nocardia farcinica</i> NBRC 15,532, a gram-positive rubber-degrading bacterium, can utilize poly(<i>cis</i>-1,4-isoprene) as the sole source of carbon and energy to produce oligo-isoprene metabolites containing aldehyde and keto end groups. A homology-based search of the genome revealed a gene encoding a latex-clearing protein (Lcp). Gene disruption analysis indicated that this gene is essential for the utilization of poly(<i>cis</i>-1,4-isoprene) in this strain. Further analysis of the genome sequence identified aldehyde dehydrogenase (ALDH) genes as potential candidates for oxidative degradation of oligo-isoprene aldehydes. Based on the enzymatic activity of the ALDH candidates, NF2_RS14000 and NF2_RS14385 may be involved in the degradation of oligo-isoprene aldehydes. Analysis of the reaction products revealed that these ALDHs oxidized tri- to penta-isoprene aldehydes, which were generated by the reaction of Lcp. Based on the inability of ALDH gene deletion mutants, we concluded that NF2_RS14000 is mainly involved in the utilization of poly(<i>cis</i>-1,4-isoprene) and the oxidative degradation of oligo-isoprene aldehydes in <i>Nocardia farcinica</i> NBRC 15,532."],"journal":["Microorganisms"],"pubmed_title":["Characterization of Latex-Clearing Protein and Aldehyde Dehydrogenases Involved in the Utilization of poly(cis-1,4-isoprene) by Nocardia farcinica NBRC 15532"],"pmcid":["PMC9782182"],"funding_grant_id":["Moonshot Research and Development Program","JPMJMI19E6"],"pubmed_authors":["Suda D","Kasai D","Anh TK","Suzuki N","Ngan NTT","Huong NL","Gibu N"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of Latex-Clearing Protein and Aldehyde Dehydrogenases Involved in the Utilization of poly(cis-1,4-isoprene) by Nocardia farcinica NBRC 15532","description":"Microbial degradation of natural rubber and synthetic poly(<i>cis</i>-1,4-isoprene) is expected to become an alternative treatment system for waste from poly(<i>cis</i>-1,4-isoprene) products including scrap tires. <i>Nocardia farcinica</i> NBRC 15,532, a gram-positive rubber-degrading bacterium, can utilize poly(<i>cis</i>-1,4-isoprene) as the sole source of carbon and energy to produce oligo-isoprene metabolites containing aldehyde and keto end groups. A homology-based search of the genome revealed a gene encoding a latex-clearing protein (Lcp). Gene disruption analysis indicated that this gene is essential for the utilization of poly(<i>cis</i>-1,4-isoprene) in this strain. Further analysis of the genome sequence identified aldehyde dehydrogenase (ALDH) genes as potential candidates for oxidative degradation of oligo-isoprene aldehydes. Based on the enzymatic activity of the ALDH candidates, NF2_RS14000 and NF2_RS14385 may be involved in the degradation of oligo-isoprene aldehydes. Analysis of the reaction products revealed that these ALDHs oxidized tri- to penta-isoprene aldehydes, which were generated by the reaction of Lcp. Based on the inability of ALDH gene deletion mutants, we concluded that NF2_RS14000 is mainly involved in the utilization of poly(<i>cis</i>-1,4-isoprene) and the oxidative degradation of oligo-isoprene aldehydes in <i>Nocardia farcinica</i> NBRC 15,532.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-06-22T03:19:16.553Z","creation":"2025-02-19T03:00:57.426Z"},"accession":"S-EPMC9782182","cross_references":{"pubmed":["36557577"],"doi":["10.3390/microorganisms10122324"]}}