biostudies-literatureSuzuki NMoonshot Research and Development Program of the New Energy and Industrial Technology Development Organization, JapanJapan Science and Technology AgencyNew Energy and Industrial Technology Development Organization2324https://www.ebi.ac.uk/biostudies/studies/S-EPMC9782182biostudies-literatureUnknown10(12)Microbial degradation of natural rubber and synthetic poly(<i>cis</i>-1,4-isoprene) is expected to become an alternative treatment system for waste from poly(<i>cis</i>-1,4-isoprene) products including scrap tires. <i>Nocardia farcinica</i> NBRC 15,532, a gram-positive rubber-degrading bacterium, can utilize poly(<i>cis</i>-1,4-isoprene) as the sole source of carbon and energy to produce oligo-isoprene metabolites containing aldehyde and keto end groups. A homology-based search of the genome revealed a gene encoding a latex-clearing protein (Lcp). Gene disruption analysis indicated that this gene is essential for the utilization of poly(<i>cis</i>-1,4-isoprene) in this strain. Further analysis of the genome sequence identified aldehyde dehydrogenase (ALDH) genes as potential candidates for oxidative degradation of oligo-isoprene aldehydes. Based on the enzymatic activity of the ALDH candidates, NF2_RS14000 and NF2_RS14385 may be involved in the degradation of oligo-isoprene aldehydes. Analysis of the reaction products revealed that these ALDHs oxidized tri- to penta-isoprene aldehydes, which were generated by the reaction of Lcp. Based on the inability of ALDH gene deletion mutants, we concluded that NF2_RS14000 is mainly involved in the utilization of poly(<i>cis</i>-1,4-isoprene) and the oxidative degradation of oligo-isoprene aldehydes in <i>Nocardia farcinica</i> NBRC 15,532.MicroorganismsCharacterization of Latex-Clearing Protein and Aldehyde Dehydrogenases Involved in the Utilization of poly(cis-1,4-isoprene) by Nocardia farcinica NBRC 15532PMC9782182Moonshot Research and Development ProgramJPMJMI19E6Suda DKasai DAnh TKSuzuki NNgan NTTHuong NLGibu NfalseCharacterization of Latex-Clearing Protein and Aldehyde Dehydrogenases Involved in the Utilization of poly(cis-1,4-isoprene) by Nocardia farcinica NBRC 15532Microbial degradation of natural rubber and synthetic poly(<i>cis</i>-1,4-isoprene) is expected to become an alternative treatment system for waste from poly(<i>cis</i>-1,4-isoprene) products including scrap tires. <i>Nocardia farcinica</i> NBRC 15,532, a gram-positive rubber-degrading bacterium, can utilize poly(<i>cis</i>-1,4-isoprene) as the sole source of carbon and energy to produce oligo-isoprene metabolites containing aldehyde and keto end groups. A homology-based search of the genome revealed a gene encoding a latex-clearing protein (Lcp). Gene disruption analysis indicated that this gene is essential for the utilization of poly(<i>cis</i>-1,4-isoprene) in this strain. Further analysis of the genome sequence identified aldehyde dehydrogenase (ALDH) genes as potential candidates for oxidative degradation of oligo-isoprene aldehydes. Based on the enzymatic activity of the ALDH candidates, NF2_RS14000 and NF2_RS14385 may be involved in the degradation of oligo-isoprene aldehydes. Analysis of the reaction products revealed that these ALDHs oxidized tri- to penta-isoprene aldehydes, which were generated by the reaction of Lcp. Based on the inability of ALDH gene deletion mutants, we concluded that NF2_RS14000 is mainly involved in the utilization of poly(<i>cis</i>-1,4-isoprene) and the oxidative degradation of oligo-isoprene aldehydes in <i>Nocardia farcinica</i> NBRC 15,532.2022-01-01T00:00:00Z2022 Nov2026-06-22T03:19:16.553Z2025-02-19T03:00:57.426ZS-EPMC97821823655757710.3390/microorganisms10122324