{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Feng C"],"funding":["California State University","National Institute of Neurological Disorders and Stroke","National Institutes of Health","National Science Foundation"],"pagination":["3547-3553"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9782390"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(24)"],"pubmed_abstract":["Parkinson's disease (PD) is the second-most prevalent neurodegenerative disorder in the U.S. α-Synuclein (α-Syn) preformed fibrils (PFFs) have been shown to propagate PD pathology in neuronal populations. However, little work has directly characterized the morphological changes on membranes associated with α-Syn PFFs at a cellular level. Scanning ion conductance microscopy (SICM) is a noninvasive <i>in situ</i> cell imaging technique and therefore uniquely advantageous to investigate PFF-induced membrane changes in neuroblastoma cells. The present work used SICM to monitor cytoplasmic membrane changes of SH-SY5Y neuroblastoma cells after incubation with varying concentrations of α-Syn PFFs. Cell membrane roughness significantly increased as the concentration of α-Syn PFFs increased. Notice"],"journal":["ACS chemical neuroscience"],"pubmed_title":["Observation of α-Synuclein Preformed Fibrils Interacting with SH-SY5Y Neuroblastoma Cell Membranes Using Scanning Ion Conductance Microscopy."],"pmcid":["PMC9782390"],"funding_grant_id":["T34GM08228","1R15NS120157-01","HRD-2112554","HRD-1547723"],"pubmed_authors":["Anguiano A","Dhoj C","Belleca S","Flores M","Garcia A","Parres-Gold J","Wang Y","Feng C","Abbas DA","Porter E"],"additional_accession":[]},"is_claimable":false,"name":"Observation of α-Synuclein Preformed Fibrils Interacting with SH-SY5Y Neuroblastoma Cell Membranes Using Scanning Ion Conductance Microscopy.","description":"Parkinson's disease (PD) is the second-most prevalent neurodegenerative disorder in the U.S. α-Synuclein (α-Syn) preformed fibrils (PFFs) have been shown to propagate PD pathology in neuronal populations. However, little work has directly characterized the morphological changes on membranes associated with α-Syn PFFs at a cellular level. Scanning ion conductance microscopy (SICM) is a noninvasive <i>in situ</i> cell imaging technique and therefore uniquely advantageous to investigate PFF-induced membrane changes in neuroblastoma cells. The present work used SICM to monitor cytoplasmic membrane changes of SH-SY5Y neuroblastoma cells after incubation with varying concentrations of α-Syn PFFs. Cell membrane roughness significantly increased as the concentration of α-Syn PFFs increased. Notice","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-04T08:00:38.496Z","creation":"2025-04-04T08:00:38.496Z"},"accession":"S-EPMC9782390","cross_references":{"pubmed":["36455298"],"doi":["10.1021/acschemneuro.2c00478"]}}