biostudies-literatureFeng CCalifornia State UniversityNational Institute of Neurological Disorders and StrokeNational Institutes of HealthNational Science Foundation3547-3553https://www.ebi.ac.uk/biostudies/studies/S-EPMC9782390biostudies-literatureUnknown13(24)Parkinson's disease (PD) is the second-most prevalent neurodegenerative disorder in the U.S. α-Synuclein (α-Syn) preformed fibrils (PFFs) have been shown to propagate PD pathology in neuronal populations. However, little work has directly characterized the morphological changes on membranes associated with α-Syn PFFs at a cellular level. Scanning ion conductance microscopy (SICM) is a noninvasive <i>in situ</i> cell imaging technique and therefore uniquely advantageous to investigate PFF-induced membrane changes in neuroblastoma cells. The present work used SICM to monitor cytoplasmic membrane changes of SH-SY5Y neuroblastoma cells after incubation with varying concentrations of α-Syn PFFs. Cell membrane roughness significantly increased as the concentration of α-Syn PFFs increased. NoticeACS chemical neuroscienceObservation of α-Synuclein Preformed Fibrils Interacting with SH-SY5Y Neuroblastoma Cell Membranes Using Scanning Ion Conductance Microscopy.PMC9782390T34GM082281R15NS120157-01HRD-2112554HRD-1547723Anguiano ADhoj CBelleca SFlores MGarcia AParres-Gold JWang YFeng CAbbas DAPorter EfalseObservation of α-Synuclein Preformed Fibrils Interacting with SH-SY5Y Neuroblastoma Cell Membranes Using Scanning Ion Conductance Microscopy.Parkinson's disease (PD) is the second-most prevalent neurodegenerative disorder in the U.S. α-Synuclein (α-Syn) preformed fibrils (PFFs) have been shown to propagate PD pathology in neuronal populations. However, little work has directly characterized the morphological changes on membranes associated with α-Syn PFFs at a cellular level. Scanning ion conductance microscopy (SICM) is a noninvasive <i>in situ</i> cell imaging technique and therefore uniquely advantageous to investigate PFF-induced membrane changes in neuroblastoma cells. The present work used SICM to monitor cytoplasmic membrane changes of SH-SY5Y neuroblastoma cells after incubation with varying concentrations of α-Syn PFFs. Cell membrane roughness significantly increased as the concentration of α-Syn PFFs increased. Notice2022-01-01T00:00:00Z2022 Dec2025-04-04T08:00:38.496Z2025-04-04T08:00:38.496ZS-EPMC97823903645529810.1021/acschemneuro.2c00478