biostudies-literatureUnknown10(12)Castillo-Alfonso FThe genera Bacillus belongs to the group of microorganisms that are known as plant growth-promoting bacteria, their metabolism has evolved to produce molecules that benefit the growth of the plant, and the production of 3-indole acetic acid (IAA) is part of its secondary metabolism. In this work, Bacillus subtilis was cultivated in a bioreactor to produce IAA using propionate and glucose as carbon sources in an M9-modified media; in both cases, tryptophan was added as a co-substrate. The yield of IAA using propionate is 17% higher compared to glucose. After 48 h of cultivation, the final concentration was 310 mg IAA/L using propionate and 230 mg IAA/L using glucose, with a concentration of 500 mg Trp/L. To gain more insight into propionate metabolism and its advantages, the genomeMicroorganisms2352https://www.ebi.ac.uk/biostudies/studies/S-EPMC9782769biostudies-literatureAnalysis of the Propionate Metabolism in Bacillus subtilis during 3-Indolacetic Production.PMC9782769Sales-Cruz AMRosales-Colunga LMQuintana-Menendez ACastillo-Alfonso FVigueras-Ramirez GOlivares-Hernandez RfalseAnalysis of the Propionate Metabolism in Bacillus subtilis during 3-Indolacetic Production.The genera Bacillus belongs to the group of microorganisms that are known as plant growth-promoting bacteria, their metabolism has evolved to produce molecules that benefit the growth of the plant, and the production of 3-indole acetic acid (IAA) is part of its secondary metabolism. In this work, Bacillus subtilis was cultivated in a bioreactor to produce IAA using propionate and glucose as carbon sources in an M9-modified media; in both cases, tryptophan was added as a co-substrate. The yield of IAA using propionate is 17% higher compared to glucose. After 48 h of cultivation, the final concentration was 310 mg IAA/L using propionate and 230 mg IAA/L using glucose, with a concentration of 500 mg Trp/L. To gain more insight into propionate metabolism and its advantages, the genome2022-01-01T00:00:00Z2022 Nov2025-04-18T17:14:15.164Z2025-04-07T04:47:06.767ZS-EPMC97827693655760510.3390/microorganisms10122352