biostudies-literatureLi KSail Plan" from Beijing Hospital AuthorityBeijing Key LaboratoryBeijing You'An HospitalBeijing Municipal Science & Technology CommissionCapital's Funds of Health Improvement and ResearchNational Key Research and Development Project8680https://www.ebi.ac.uk/biostudies/studies/S-EPMC9782793biostudies-literatureUnknown27(24)Neuroblast differentiation-associated protein AHNAK, a large structural scaffold protein, remains mysterious in biological processes. AHNAK plays a suppressive or progressive role in different types of cancers. To investigate the role of the AHNAK in hepatocellular carcinoma (HCC), cell viability assays were performed to determine the cell proliferation of the stable AHNAK-knockdown HepG2 cell line; co-immunoprecipitation (Co-IP) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) were performed on HCC and matched paracancerous (MPC) tissues. The Metascape platform was used for enrichment analyses; the "ComplexHeatmap" package was applied for cluster analyses and visualization. Co-IP, Western botting and immunofluorescence double staining were performed to assess theMolecules (Basel, Switzerland)AHNAK Contributes to Hepatocellular Carcinoma Growth by Interacting with IGF-1R.PMC9782793Z171100001017078CFH2020-1-2182BJYAHKF2021003,YNKTQN2021007XMLX2017112020YFE0202400BZ0373Sun LSong KZhang YTian YLi AWang HLi KHou YfalseAHNAK Contributes to Hepatocellular Carcinoma Growth by Interacting with IGF-1R.Neuroblast differentiation-associated protein AHNAK, a large structural scaffold protein, remains mysterious in biological processes. AHNAK plays a suppressive or progressive role in different types of cancers. To investigate the role of the AHNAK in hepatocellular carcinoma (HCC), cell viability assays were performed to determine the cell proliferation of the stable AHNAK-knockdown HepG2 cell line; co-immunoprecipitation (Co-IP) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) were performed on HCC and matched paracancerous (MPC) tissues. The Metascape platform was used for enrichment analyses; the "ComplexHeatmap" package was applied for cluster analyses and visualization. Co-IP, Western botting and immunofluorescence double staining were performed to assess the2022-01-01T00:00:00Z2022 Dec2025-04-04T07:59:04.071Z2025-04-04T07:59:04.071ZS-EPMC97827933655781310.3390/molecules27248680