biostudies-literatureDalal SBLRD VANHLBI NIH HHSNational Institutes of HealthDepartment of Veterans AffairsNational Heart Lung and Blood Institute121216https://www.ebi.ac.uk/biostudies/studies/S-EPMC9784153biostudies-literatureUnknown312<h4>Aims</h4>Pretreatment with ubiquitin (UB) associates with preservation of heart function 3 days post-ischemia/reperfusion (I/R) injury. This study investigated the cardioprotective potential of exogenous UB late after myocardial I/R injury. To enhance the clinical relevance, UB treatment was started at the time of reperfusion and continued for 28 days post-I/R.<h4>Main methods</h4>Mice underwent ligation of the left anterior descending coronary artery for 45 min. At the time of reperfusion, mice were treated with UB or saline which was continued until 28 days post-I/R. Heart function was measured at 3, 7, 14 and 28 days post-I/R using echocardiography. Biochemical parameters of the heart and serum cytokines/chemokines levels were measured 28 days post-I/R.<h4>Key findings</h4>I/R decreLife sciencesPost-ischemic cardioprotective potential of exogenous ubiquitin in myocardial remodeling late after ischemia/reperfusion injury.PMC9784153R15 HL141947I01 BX004045R21 HL092459R15 HL156214Dalal SSingh KShook PLSingh MfalsePost-ischemic cardioprotective potential of exogenous ubiquitin in myocardial remodeling late after ischemia/reperfusion injury.<h4>Aims</h4>Pretreatment with ubiquitin (UB) associates with preservation of heart function 3 days post-ischemia/reperfusion (I/R) injury. This study investigated the cardioprotective potential of exogenous UB late after myocardial I/R injury. To enhance the clinical relevance, UB treatment was started at the time of reperfusion and continued for 28 days post-I/R.<h4>Main methods</h4>Mice underwent ligation of the left anterior descending coronary artery for 45 min. At the time of reperfusion, mice were treated with UB or saline which was continued until 28 days post-I/R. Heart function was measured at 3, 7, 14 and 28 days post-I/R using echocardiography. Biochemical parameters of the heart and serum cytokines/chemokines levels were measured 28 days post-I/R.<h4>Key findings</h4>I/R decre2023-01-01T00:00:00Z2023 Jan2025-04-20T09:18:56.182Z2025-04-20T09:18:56.182ZS-EPMC97841533643522510.1016/j.lfs.2022.121216