biostudies-literatureAddo PWNatural Sciences and Engineering Research Council Collaborative Research and DevelopmentCollaborative Research and Training Experience-Quality Assurance and Quality Control for Cannabis Production8803https://www.ebi.ac.uk/biostudies/studies/S-EPMC9784742biostudies-literatureUnknown27(24)Limited studies have explored different extraction techniques that improve cannabis extraction with scale-up potential. Ultrasound-assisted and microwave-assisted extraction were evaluated to maximize the yield and concentration of cannabinoids and terpenes. A central composite rotatable design was used to optimize independent factors (sample-to-solvent ratio, extraction time, extraction temperature, and duty cycle). The optimal conditions for ultrasound- and microwave-assisted extraction were the sample-to-solvent ratios of 1:15 and 1:14.4, respectively, for 30 min at 60 °C. Ultrasound-assisted extraction yielded 14.4% and 14.2% more oil and terpenes, respectively, compared with microwave-assisted extracts. Ultrasound-assisted extraction increased cannabinoid concentration from 13.2-39.2%Molecules (Basel, Switzerland)Microwave- and Ultrasound-Assisted Extraction of Cannabinoids and Terpenes from Cannabis Using Response Surface Methodology.PMC9784742NSERC CRDPJ 543704-19CREATE CREAT/543319- 2020Raghavan VMacPherson SAddo PWLefsrud MMcRae GBilodeau SEParis MSagili SUKRGladu-Gallant FABates JOrsat VMacKenzie DAfalseMicrowave- and Ultrasound-Assisted Extraction of Cannabinoids and Terpenes from Cannabis Using Response Surface Methodology.Limited studies have explored different extraction techniques that improve cannabis extraction with scale-up potential. Ultrasound-assisted and microwave-assisted extraction were evaluated to maximize the yield and concentration of cannabinoids and terpenes. A central composite rotatable design was used to optimize independent factors (sample-to-solvent ratio, extraction time, extraction temperature, and duty cycle). The optimal conditions for ultrasound- and microwave-assisted extraction were the sample-to-solvent ratios of 1:15 and 1:14.4, respectively, for 30 min at 60 °C. Ultrasound-assisted extraction yielded 14.4% and 14.2% more oil and terpenes, respectively, compared with microwave-assisted extracts. Ultrasound-assisted extraction increased cannabinoid concentration from 13.2-39.2%2022-01-01T00:00:00Z2022 Dec2025-04-04T08:00:16.442Z2025-04-04T08:00:16.442ZS-EPMC97847423655794910.3390/molecules27248803