biostudies-literatureUnknown11(24)Ruksiriwanich WLeaves of guava (<i>Psidium guajava</i> L.) have been used in Thai folk medicine without any supporting evidence as a traditional herbal remedy for hair loss. Androgenetic alopecia (AGA) is chronic hair loss caused by effects of androgens in those with a genetic predisposition, resulting in hair follicle miniaturization. Our objectives were to provide the mechanistic assessment of guava leaf extract on gene expressions related to the androgen pathway in well-known in vitro models, hair follicle dermal papilla cells (HFDPC), and human prostate cancer cells (DU-145), and to determine its bioactive constituents and antioxidant activities. LC-MS analysis demonstrated that the main components of the ethanolic extract of guava leaves are phenolic substances, specifically catechin, gallic acid, aPlants (Basel, Switzerland)3514https://www.ebi.ac.uk/biostudies/studies/S-EPMC9784754biostudies-literatureGuava (<i>Psidium guajava</i> L.) Leaf Extract as Bioactive Substances for Anti-Androgen and Antioxidant Activities.PMC9784754Jantanasakulwong KSringarm KKhantham CBoonpisuttinant KRuksiriwanich WRachtanapun PSommano SRJantrawut PBarba FJChittasupho CChutoprapat RPhimolsiripol YMuangsanguan AfalseGuava (<i>Psidium guajava</i> L.) Leaf Extract as Bioactive Substances for Anti-Androgen and Antioxidant Activities.Leaves of guava (<i>Psidium guajava</i> L.) have been used in Thai folk medicine without any supporting evidence as a traditional herbal remedy for hair loss. Androgenetic alopecia (AGA) is chronic hair loss caused by effects of androgens in those with a genetic predisposition, resulting in hair follicle miniaturization. Our objectives were to provide the mechanistic assessment of guava leaf extract on gene expressions related to the androgen pathway in well-known in vitro models, hair follicle dermal papilla cells (HFDPC), and human prostate cancer cells (DU-145), and to determine its bioactive constituents and antioxidant activities. LC-MS analysis demonstrated that the main components of the ethanolic extract of guava leaves are phenolic substances, specifically catechin, gallic acid, a2022-01-01T00:00:00Z2022 Dec2025-04-18T15:59:09.164Z2025-04-07T02:51:58.209ZS-EPMC97847543655962610.3390/plants11243514