biostudies-literatureAkomoneh EASOFI 20212497https://www.ebi.ac.uk/biostudies/studies/S-EPMC9787740biostudies-literatureUnknown10(12)With increasing incidence of pathogenic <i>Neisseria</i> infections coupled with emerging resistance to antimicrobials, alternative approaches to limit the spread are sought. We investigated the inhibitory effect of oropharyngeal microbiota on the growth of <i>N. gonorrhoeae</i> and <i>N. meningitidis</i> and the impact of the essential oil-based mouthwash Listerine Cool Mint^® (Listerine). Oropharyngeal swabs from 64 men who have sex with men (<i>n</i> = 118) from a previous study (PReGo study) were analysed (ClinicalTrials.gov, NCT03881007). These included 64 baseline and 54 samples following three months of daily use of Listerine. Inhibition was confirmed by agar overlay assay, and inhibitory bacteria isolated using replica plating and identified using MALDI-TOF. The number of inhibitory isolates were compared before and after Listerine use. Thirty-one pharyngeal samples (26%) showed inhibitory activity against <i>N. gonorrhoeae</i> and/or <i>N. meningitidis</i>, and 62 inhibitory isolates were characterised. Fourteen species belonging to the genera <i>Streptococci</i> and <i>Rothia</i> were identified. More inhibitory isolates were observed following Listerine use compared to baseline, although this effect was not statistically significant (<i>p</i> = 0.073). This study isolated and identified inhibitory bacteria against pathogenic <i>Neisseria</i> spp. and established that daily Listerine use did not decrease their prevalence. These findings could provide a new approach for the prevention and treatment of pharyngeal <i>Neisseria</i> infections.MicroorganismsThe Discovery of Oropharyngeal Microbiota with Inhibitory Activity against Pathogenic <i>Neisseria gonorrhoeae</i> and <i>Neisseria meningitidis</i>: An In Vitro Study of Clinical Isolates.PMC9787740SOFI 2021Manoharan-Basil SSBritto XBAkomoneh EAKenyon CVanbaelen TLaumen JGEAbdellati SVan Dijck CfalseThe Discovery of Oropharyngeal Microbiota with Inhibitory Activity against Pathogenic <i>Neisseria gonorrhoeae</i> and <i>Neisseria meningitidis</i>: An In Vitro Study of Clinical Isolates.With increasing incidence of pathogenic <i>Neisseria</i> infections coupled with emerging resistance to antimicrobials, alternative approaches to limit the spread are sought. We investigated the inhibitory effect of oropharyngeal microbiota on the growth of <i>N. gonorrhoeae</i> and <i>N. meningitidis</i> and the impact of the essential oil-based mouthwash Listerine Cool Mint^® (Listerine). Oropharyngeal swabs from 64 men who have sex with men (<i>n</i> = 118) from a previous study (PReGo study) were analysed (ClinicalTrials.gov, NCT03881007). These included 64 baseline and 54 samples following three months of daily use of Listerine. Inhibition was confirmed by agar overlay assay, and inhibitory bacteria isolated using replica plating and identified using MALDI-TOF. The number of inhibitory isolates were compared before and after Listerine use. Thirty-one pharyngeal samples (26%) showed inhibitory activity against <i>N. gonorrhoeae</i> and/or <i>N. meningitidis</i>, and 62 inhibitory isolates were characterised. Fourteen species belonging to the genera <i>Streptococci</i> and <i>Rothia</i> were identified. More inhibitory isolates were observed following Listerine use compared to baseline, although this effect was not statistically significant (<i>p</i> = 0.073). This study isolated and identified inhibitory bacteria against pathogenic <i>Neisseria</i> spp. and established that daily Listerine use did not decrease their prevalence. These findings could provide a new approach for the prevention and treatment of pharyngeal <i>Neisseria</i> infections.2022-01-01T00:00:00Z2022 Dec2026-04-08T11:09:05.197Z2025-02-19T03:24:33.658ZS-EPMC97877403655775010.3390/microorganisms10122497