{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Langsdorf A"],"funding":["Federal Ministry of Education and Research"],"pagination":["8684"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9788380"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(24)"],"pubmed_abstract":["Green waste, e.g., grass clippings, is currently insufficiently recycled and has untapped potential as a valuable resource. Our aim was to use juice from grass clippings as a growth medium for microorganisms. Herein, we demonstrate the production of the sesquiterpene α-humulene with the versatile organism <i>Cupriavidus necator</i> pKR-hum on a growth medium from grass clippings. The medium was compared with established media in terms of microbial growth and terpene production. <i>C. necator</i> pKR-hum shows a maximum growth rate of 0.43 h<sup>-1</sup> in the grass medium and 0.50 h<sup>-1</sup> in a lysogeny broth (LB) medium. With the grass medium, 2 mg/L of α-humulene were produced compared to 10 mg/L with the LB medium. By concentrating the grass medium and using a controlled bioreact"],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium."],"pmcid":["PMC9788380"],"funding_grant_id":["031B0903B"],"pubmed_authors":["Drommershausen AL","Volkmar M","Holtmann D","Langsdorf A","Ulber R"],"additional_accession":[]},"is_claimable":false,"name":"Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium.","description":"Green waste, e.g., grass clippings, is currently insufficiently recycled and has untapped potential as a valuable resource. Our aim was to use juice from grass clippings as a growth medium for microorganisms. Herein, we demonstrate the production of the sesquiterpene α-humulene with the versatile organism <i>Cupriavidus necator</i> pKR-hum on a growth medium from grass clippings. The medium was compared with established media in terms of microbial growth and terpene production. <i>C. necator</i> pKR-hum shows a maximum growth rate of 0.43 h<sup>-1</sup> in the grass medium and 0.50 h<sup>-1</sup> in a lysogeny broth (LB) medium. With the grass medium, 2 mg/L of α-humulene were produced compared to 10 mg/L with the LB medium. By concentrating the grass medium and using a controlled bioreact","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-18T15:59:33.656Z","creation":"2024-11-19T15:22:05.811Z"},"accession":"S-EPMC9788380","cross_references":{"pubmed":["36557817"],"doi":["10.3390/molecules27248684"]}}