biostudies-literatureLangsdorf AFederal Ministry of Education and Research8684https://www.ebi.ac.uk/biostudies/studies/S-EPMC9788380biostudies-literatureUnknown27(24)Green waste, e.g., grass clippings, is currently insufficiently recycled and has untapped potential as a valuable resource. Our aim was to use juice from grass clippings as a growth medium for microorganisms. Herein, we demonstrate the production of the sesquiterpene α-humulene with the versatile organism <i>Cupriavidus necator</i> pKR-hum on a growth medium from grass clippings. The medium was compared with established media in terms of microbial growth and terpene production. <i>C. necator</i> pKR-hum shows a maximum growth rate of 0.43 h^-1 in the grass medium and 0.50 h^-1 in a lysogeny broth (LB) medium. With the grass medium, 2 mg/L of α-humulene were produced compared to 10 mg/L with the LB medium. By concentrating the grass medium and using a controlled bioreactMolecules (Basel, Switzerland)Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium.PMC9788380031B0903BDrommershausen ALVolkmar MHoltmann DLangsdorf AUlber RfalseFermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium.Green waste, e.g., grass clippings, is currently insufficiently recycled and has untapped potential as a valuable resource. Our aim was to use juice from grass clippings as a growth medium for microorganisms. Herein, we demonstrate the production of the sesquiterpene α-humulene with the versatile organism <i>Cupriavidus necator</i> pKR-hum on a growth medium from grass clippings. The medium was compared with established media in terms of microbial growth and terpene production. <i>C. necator</i> pKR-hum shows a maximum growth rate of 0.43 h^-1 in the grass medium and 0.50 h^-1 in a lysogeny broth (LB) medium. With the grass medium, 2 mg/L of α-humulene were produced compared to 10 mg/L with the LB medium. By concentrating the grass medium and using a controlled bioreact2022-01-01T00:00:00Z2022 Dec2025-04-18T15:59:33.656Z2024-11-19T15:22:05.811ZS-EPMC97883803655781710.3390/molecules27248684