{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["479(24)"],"submitter":["Dietrich MH"],"pubmed_abstract":["Transmission blocking interventions can stop malaria parasite transmission from mosquito to human by inhibiting parasite infection in mosquitos. One of the most advanced candidates for a malaria transmission blocking vaccine is Pfs230. Pfs230 is the largest member of the 6-cysteine protein family with 14 consecutive 6-cysteine domains and is expressed on the surface of gametocytes and gametes. Here, we present the crystal structure of the first two 6-cysteine domains of Pfs230. We identified high affinity Pfs230-specific nanobodies that recognized gametocytes and bind to distinct sites on Pfs230, which were isolated from immunized alpacas. Using two non-overlapping Pfs230 nanobodies, we show that these nanobodies significantly blocked P. falciparum transmission and reduced the formation of"],"journal":["The Biochemical journal"],"pagination":["2529-2546"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9788556"],"repository":["biostudies-literature"],"pubmed_title":["Nanobodies against Pfs230 block Plasmodium falciparum transmission."],"pmcid":["PMC9788556"],"pubmed_authors":["Keremane S","Adair A","Cowman AF","Chan LJ","Healer J","Lopaticki S","Reaksudsan K","Dixon MWA","O'Neill MT","Dietrich MH","Trickey S","Gabriela M","Tham WH","Tan LL"],"additional_accession":[]},"is_claimable":false,"name":"Nanobodies against Pfs230 block Plasmodium falciparum transmission.","description":"Transmission blocking interventions can stop malaria parasite transmission from mosquito to human by inhibiting parasite infection in mosquitos. One of the most advanced candidates for a malaria transmission blocking vaccine is Pfs230. Pfs230 is the largest member of the 6-cysteine protein family with 14 consecutive 6-cysteine domains and is expressed on the surface of gametocytes and gametes. Here, we present the crystal structure of the first two 6-cysteine domains of Pfs230. We identified high affinity Pfs230-specific nanobodies that recognized gametocytes and bind to distinct sites on Pfs230, which were isolated from immunized alpacas. Using two non-overlapping Pfs230 nanobodies, we show that these nanobodies significantly blocked P. falciparum transmission and reduced the formation of","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-05-27T21:03:23.1Z","creation":"2025-02-18T22:34:35.487Z"},"accession":"S-EPMC9788556","cross_references":{"pubmed":["36520108"],"doi":["10.1042/BCJ20220554"]}}