{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Peixoto MM"],"funding":["Agence Nationale de la Recherche","FCT/MCTES","Institut National de la Santé et de la Recherche Médicale","Institut Pasteur"],"pagination":["960-969"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9790487"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["101(11)"],"pubmed_abstract":["The fetal liver (FL) is the main hematopoietic organ during embryonic development. The FL is also the unique anatomical site where hematopoietic stem cells expand before colonizing the bone marrow, where they ensure life-long blood cell production and become mostly resting. The identification of the different cell types that comprise the hematopoietic stroma in the FL is essential to understand the signals required for the expansion and differentiation of the hematopoietic stem cells. We used a panel of monoclonal antibodies to identify FL stromal cells in a 5-laser equipped spectral flow cytometry (FCM) analyzer. The \"Autofluorescence Finder\" of SONY ID7000 software identified two distinct autofluorescence emission spectra. Using autofluorescence as a fluorescence parameter we could assig"],"journal":["Cytometry. Part A : the journal of the International Society for Analytical Cytology"],"pubmed_title":["Identification of fetal liver stroma in spectral cytometry using the parameter autofluorescence."],"pmcid":["PMC9790487"],"funding_grant_id":["EPIDEV","DELSTAR","Twothyme","PTDC/MED-OUT/32656/2017 (POCI-01-0145-FEDER-032656","Core funding"],"pubmed_authors":["Soares-da-Silva F","Novault S","Peixoto MM","Ait-Mansour C","Mailhe MP","Schmutz S","Cumano A"],"additional_accession":[]},"is_claimable":false,"name":"Identification of fetal liver stroma in spectral cytometry using the parameter autofluorescence.","description":"The fetal liver (FL) is the main hematopoietic organ during embryonic development. The FL is also the unique anatomical site where hematopoietic stem cells expand before colonizing the bone marrow, where they ensure life-long blood cell production and become mostly resting. The identification of the different cell types that comprise the hematopoietic stroma in the FL is essential to understand the signals required for the expansion and differentiation of the hematopoietic stem cells. We used a panel of monoclonal antibodies to identify FL stromal cells in a 5-laser equipped spectral flow cytometry (FCM) analyzer. The \"Autofluorescence Finder\" of SONY ID7000 software identified two distinct autofluorescence emission spectra. Using autofluorescence as a fluorescence parameter we could assig","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-04T12:10:08.331Z","creation":"2025-04-04T12:10:08.331Z"},"accession":"S-EPMC9790487","cross_references":{"pubmed":["35491762"],"doi":["10.1002/cyto.a.24567"]}}