biostudies-literatureUnknown43(3)Yoshida HGraduate School of Infection Control Sciences &amp; Ōmura Satoshi Memorial Institute<h4>Background</h4>Comparative analysis of virulence factors (VFs) between <i>Pasteurella canis</i> and <i>Pasteurella multocida</i> are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of <i>P. canis</i> and assessed the toxin gene prevalence unique to <i>P. canis</i> among clinical isolates of <i>P. canis</i> and <i>P. multocida</i>.<h4>Methods</h4>We selected 10 <i>P. canis</i> and 16 <i>P. multocida</i> whole-genome sequences (WGSs) from the National Center for Biotechnology database. The VFanalyzer tool was used to estimate <i>P. canis</i>-characteristic VFs. Amino acid sequences of VFs were compared with multiple-aligned sequences. The genome structure containing <i>P. canis</i>-characteristic and adjacent loci wAnnals of laboratory medicine263-272https://www.ebi.ac.uk/biostudies/studies/S-EPMC9791007biostudies-literatureVirulence-associated Genome Sequences of <i>Pasteurella canis</i> and Unique Toxin Gene Prevalence of <i>P. canis</i> and <i>Pasteurella multocida</i> Isolated from Humans and Companion Animals.PMC9791007Kim JMTakahashi TShibata SOkuzumi KMaeda TShizuno KTsuyuki YGoto MYoshida HKim JSfalseVirulence-associated Genome Sequences of <i>Pasteurella canis</i> and Unique Toxin Gene Prevalence of <i>P. canis</i> and <i>Pasteurella multocida</i> Isolated from Humans and Companion Animals.<h4>Background</h4>Comparative analysis of virulence factors (VFs) between <i>Pasteurella canis</i> and <i>Pasteurella multocida</i> are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of <i>P. canis</i> and assessed the toxin gene prevalence unique to <i>P. canis</i> among clinical isolates of <i>P. canis</i> and <i>P. multocida</i>.<h4>Methods</h4>We selected 10 <i>P. canis</i> and 16 <i>P. multocida</i> whole-genome sequences (WGSs) from the National Center for Biotechnology database. The VFanalyzer tool was used to estimate <i>P. canis</i>-characteristic VFs. Amino acid sequences of VFs were compared with multiple-aligned sequences. The genome structure containing <i>P. canis</i>-characteristic and adjacent loci w2023-01-01T00:00:00Z2023 May2025-04-05T11:23:45.56Z2025-04-05T11:23:45.56ZS-EPMC97910073654433810.3343/alm.2023.43.3.263