biostudies-literatureUnknown11(12)McGrath FMFood Allergy and Anaphylaxis NetworkRoyal Perth Hospital Medical Research FoundationAustralian Genome Research Facility<h4>Objective</h4>Mechanisms underlying the anaphylactic reaction in humans are not fully understood. Here, we aimed at improving our understanding of anaphylaxis by investigating gene expression changes.<h4>Methods</h4>Microarray data set GSE69063 was analysed, describing emergency department (ED) patients with severe anaphylaxis (<i>n</i> = 12), moderate anaphylaxis (<i>n</i> = 6), sepsis (<i>n</i> = 20) and trauma (<i>n</i> = 11). Samples were taken at ED presentation (T0) and 1 h later (T1). Healthy controls were age and sex matched to ED patient groups. Gene expression changes were determined using <i>limma</i>, and pathway analysis applied. Differentially expressed genes were validated in an independent cohort of anaphylaxis patients (<i>n</i> = 31) and matched healthy controls (<i>nClinical & translational immunologye1435https://www.ebi.ac.uk/biostudies/studies/S-EPMC9791329biostudies-literatureGenes involved in platelet aggregation and activation are downregulated during acute anaphylaxis in humans.PMC9791329McGrath FMMacdonald SPWoo AJFrancis AArendts GBosio EFatovich DMfalseGenes involved in platelet aggregation and activation are downregulated during acute anaphylaxis in humans.<h4>Objective</h4>Mechanisms underlying the anaphylactic reaction in humans are not fully understood. Here, we aimed at improving our understanding of anaphylaxis by investigating gene expression changes.<h4>Methods</h4>Microarray data set GSE69063 was analysed, describing emergency department (ED) patients with severe anaphylaxis (<i>n</i> = 12), moderate anaphylaxis (<i>n</i> = 6), sepsis (<i>n</i> = 20) and trauma (<i>n</i> = 11). Samples were taken at ED presentation (T0) and 1 h later (T1). Healthy controls were age and sex matched to ED patient groups. Gene expression changes were determined using <i>limma</i>, and pathway analysis applied. Differentially expressed genes were validated in an independent cohort of anaphylaxis patients (<i>n</i> = 31) and matched healthy controls (<i>n2022-01-01T00:00:00Z20222026-05-31T15:53:20.331Z2024-10-17T22:07:39.393ZS-EPMC97913293658315910.1002/cti2.1435