biostudies-literatureRiester OBundesministerium für Bildung und ForschungInterregHochschule Furtwangen540https://www.ebi.ac.uk/biostudies/studies/S-EPMC9793564biostudies-literatureUnknown20(1)<h4>Background</h4>In vivo-mimicking conditions are critical in in vitro cell analysis to obtain clinically relevant results. The required conditions, comparable to those prevalent in nature, can be provided by microfluidic dynamic cell cultures. Microfluidics can be used to fabricate and test the functionality and biocompatibility of newly developed nanosystems or to apply micro- and nanoelectromechanical systems embedded in a microfluidic system. However, the use of microfluidic systems is often hampered by their accessibility, acquisition cost, or customization, especially for scientists whose primary research focus is not microfluidics.<h4>Results</h4>Here we present a method for 3D printing that can be applied without special prior knowledge and sophisticated equipment to produce variJournal of nanobiotechnologyDirect 3D printed biocompatible microfluidics: assessment of human mesenchymal stem cell differentiation and cytotoxic drug screening in a dynamic culture system.PMC9793564FKZ:13FH5E01IAref: 5115/3.17Riester ODeigner HPLaufer SfalseDirect 3D printed biocompatible microfluidics: assessment of human mesenchymal stem cell differentiation and cytotoxic drug screening in a dynamic culture system.<h4>Background</h4>In vivo-mimicking conditions are critical in in vitro cell analysis to obtain clinically relevant results. The required conditions, comparable to those prevalent in nature, can be provided by microfluidic dynamic cell cultures. Microfluidics can be used to fabricate and test the functionality and biocompatibility of newly developed nanosystems or to apply micro- and nanoelectromechanical systems embedded in a microfluidic system. However, the use of microfluidic systems is often hampered by their accessibility, acquisition cost, or customization, especially for scientists whose primary research focus is not microfluidics.<h4>Results</h4>Here we present a method for 3D printing that can be applied without special prior knowledge and sophisticated equipment to produce vari2022-01-01T00:00:00Z2022 Dec2025-04-26T11:52:33.53Z2025-04-06T13:51:46.873ZS-EPMC97935643657553010.1186/s12951-022-01737-7