biostudies-literatureUnknown32(1)Kim NHG protein-coupled receptors (GPCRs) are in the spotlight as drug targets due to the fact that multiple research results have verified the correlation between the activation of GPCRs and disease indications. This is because the GPCRs are present across the cell membranes, which interact with either extracellular ligands or other types of compartments and simultaneously mediate intracellular signaling. Despite the importance of the GPCRs as drug targets, they are too difficult to express in soluble forms. Currently, the difficulty of preparing functional GPCRs and the lack of efficient antibody screening methods are the most challenging steps in the discovery of antibodies targeting GPCRs. In this study, we developed a powerful platform that facilitates isolating GPCR-specific antibodies by Protein science : a publication of the Protein Societye4520https://www.ebi.ac.uk/biostudies/studies/S-EPMC9793966biostudies-literatureA robust antibody discovery platform for difficult-to-express G protein-coupled receptors.PMC9793966Kang THKang SPark GHYu YGShim GKim NHfalseA robust antibody discovery platform for difficult-to-express G protein-coupled receptors.G protein-coupled receptors (GPCRs) are in the spotlight as drug targets due to the fact that multiple research results have verified the correlation between the activation of GPCRs and disease indications. This is because the GPCRs are present across the cell membranes, which interact with either extracellular ligands or other types of compartments and simultaneously mediate intracellular signaling. Despite the importance of the GPCRs as drug targets, they are too difficult to express in soluble forms. Currently, the difficulty of preparing functional GPCRs and the lack of efficient antibody screening methods are the most challenging steps in the discovery of antibodies targeting GPCRs. In this study, we developed a powerful platform that facilitates isolating GPCR-specific antibodies by 2023-01-01T00:00:00Z2023 Jan2025-04-26T07:51:21.818Z2025-04-06T12:27:38.605ZS-EPMC97939663641938210.1002/pro.4520