{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Miluzio A"],"funding":["Associazione Italiana per la Ricerca sul Cancro","Fondazione Romeo ed Enrica Invernizzi"],"pagination":["104390"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9795807"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["87"],"pubmed_abstract":["<h4>Background</h4>The COVID-19 pandemic is an infectious disease caused by SARS-CoV-2. The first step of SARS-CoV-2 infection is the recognition of angiotensin-converting enzyme 2 (ACE2) receptors by the receptor-binding domain (RBD) of the viral Spike (S) glycoprotein. Although the molecular and structural bases of the SARS-CoV-2-RBD/hACE2 interaction have been thoroughly investigated in vitro, the relationship between hACE2 expression and in vivo infection is less understood.<h4>Methods</h4>Here, we developed an efficient SARS-CoV-2-RBD binding assay suitable for super resolution microscopy and simultaneous hACE2 immunodetection and mapped the correlation between hACE2 receptor abundance and SARS-CoV-2-RBD binding, both in vitro and in human lung biopsies. Next, we explored the specific"],"journal":["EBioMedicine"],"pubmed_title":["Mapping of functional SARS-CoV-2 receptors in human lungs establishes differences in variant binding and SLC1A5 as a viral entry modulator of hACE2."],"pmcid":["PMC9795807"],"funding_grant_id":["26178"],"pubmed_authors":["Scagliola A","Oliveto S","Manfrini N","Pesce E","Conti M","Manganaro L","Ricciardi S","Biffo S","Cordiglieri C","Abrignani S","Fasciani A","Terracciano L","De Francesco R","Cuomo A","Toccafondi M","Donnici L","Grifantini R","Bombaci M","Miluzio A"],"additional_accession":[]},"is_claimable":false,"name":"Mapping of functional SARS-CoV-2 receptors in human lungs establishes differences in variant binding and SLC1A5 as a viral entry modulator of hACE2.","description":"<h4>Background</h4>The COVID-19 pandemic is an infectious disease caused by SARS-CoV-2. The first step of SARS-CoV-2 infection is the recognition of angiotensin-converting enzyme 2 (ACE2) receptors by the receptor-binding domain (RBD) of the viral Spike (S) glycoprotein. Although the molecular and structural bases of the SARS-CoV-2-RBD/hACE2 interaction have been thoroughly investigated in vitro, the relationship between hACE2 expression and in vivo infection is less understood.<h4>Methods</h4>Here, we developed an efficient SARS-CoV-2-RBD binding assay suitable for super resolution microscopy and simultaneous hACE2 immunodetection and mapped the correlation between hACE2 receptor abundance and SARS-CoV-2-RBD binding, both in vitro and in human lung biopsies. Next, we explored the specific","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-05-29T04:35:47.715Z","creation":"2024-12-04T03:30:31.375Z"},"accession":"S-EPMC9795807","cross_references":{"pubmed":["36584595"],"doi":["10.1016/j.ebiom.2022.104390"]}}