biostudies-literatureHjortdal JAgence Nationale de la Recherche2337-2347https://www.ebi.ac.uk/biostudies/studies/S-EPMC9796948biostudies-literatureUnknown22(10)Acute rejection (AR) of corneal transplants (CT) has a profound effect on subsequent graft survival but detailed immunological studies in human CT recipients are lacking. In this multi-site, cross-sectional study, clinical details and blood samples were collected from adults with clinically diagnosed AR of full-thickness (FT)-CT (n = 35) and posterior lamellar (PL)-CT (n = 21) along with Stable CT recipients (n = 177) and adults with non-transplanted corneal disease (n = 40). For those with AR, additional samples were collected 3 months later. Immune cell analysis was performed by whole-genome microarrays (whole blood) and high-dimensional multi-color flow cytometry (peripheral blood mononuclear cells). For both, no activation signature was identified within the B cell and T cell repertoirAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsPeripheral blood immune cell profiling of acute corneal transplant rejection.PMC9796948ANR‐10‐IBHU‐005ANR‐17‐CE17‐0008Brouard SGriffin MDPleyer UBylesjo MKarakachoff MArmitage WJHjortdal JGabhann PMGourraud PAWalkinshaw MDDegauque NVabres BCadoux MMurphy CCTole DfalsePeripheral blood immune cell profiling of acute corneal transplant rejection.Acute rejection (AR) of corneal transplants (CT) has a profound effect on subsequent graft survival but detailed immunological studies in human CT recipients are lacking. In this multi-site, cross-sectional study, clinical details and blood samples were collected from adults with clinically diagnosed AR of full-thickness (FT)-CT (n = 35) and posterior lamellar (PL)-CT (n = 21) along with Stable CT recipients (n = 177) and adults with non-transplanted corneal disease (n = 40). For those with AR, additional samples were collected 3 months later. Immune cell analysis was performed by whole-genome microarrays (whole blood) and high-dimensional multi-color flow cytometry (peripheral blood mononuclear cells). For both, no activation signature was identified within the B cell and T cell repertoir2022-01-01T00:00:00Z2022 Oct2025-04-04T10:59:08.34Z2025-04-04T10:59:08.34ZS-EPMC97969483570429010.1111/ajt.17119