biostudies-literatureDoyle ADNCATS NIH HHSNIDDK NIH HHSNIAID NIH HHSNHLBI NIH HHSNIAAA NIH HHSMayo ClinicNCI NIH HHSAmerican Partnership for Eosinophilic DisordersNational Institutes of HealthPhoenix Children&apos;s Hospital192-201https://www.ebi.ac.uk/biostudies/studies/S-EPMC9797443biostudies-literatureUnknown78(1)<h4>Background</h4>Eosinophilic esophagitis (EoE) is a chronic allergic disease associated with type 2 inflammation and epithelial barrier dysfunction. The etiology is unknown, however, genetic heritability studies suggest environmental factors play a key role in pathogenesis. Detergents, such as sodium dodecyl sulfate (SDS), are common ingredients in household products such as dish soap and toothpaste. We hypothesized detergent exposure decreases epithelial barrier function and induces esophageal inflammation.<h4>Methods</h4>Immortalized esophageal epithelial cells (EPC2) were cultured in air-liquid interface (ALI) and exposed to SDS. Barrier function/activity was assessed by transepithelial electrical resistance (TEER), FITC-dextran flux, and RT-PCR. Additionally, SDS-treated mouse esophAllergyDetergent exposure induces epithelial barrier dysfunction and eosinophilic inflammation in the esophagus.PMC9797443R01 AA026297U54 AI117804U2C TR002818R01 DK114436L30 CA264714R37 AI071106R01 AI128729P30 DK132710R01 HL117823Putikova AMasuda MYLeSuer WEDoyle ADWright BLFlashner SKita HLuo HRank MAPyon GCNakagawa HfalseDetergent exposure induces epithelial barrier dysfunction and eosinophilic inflammation in the esophagus.<h4>Background</h4>Eosinophilic esophagitis (EoE) is a chronic allergic disease associated with type 2 inflammation and epithelial barrier dysfunction. The etiology is unknown, however, genetic heritability studies suggest environmental factors play a key role in pathogenesis. Detergents, such as sodium dodecyl sulfate (SDS), are common ingredients in household products such as dish soap and toothpaste. We hypothesized detergent exposure decreases epithelial barrier function and induces esophageal inflammation.<h4>Methods</h4>Immortalized esophageal epithelial cells (EPC2) were cultured in air-liquid interface (ALI) and exposed to SDS. Barrier function/activity was assessed by transepithelial electrical resistance (TEER), FITC-dextran flux, and RT-PCR. Additionally, SDS-treated mouse esoph2023-01-01T00:00:00Z2023 Jan2025-04-21T15:08:55.255Z2025-04-21T15:08:55.255ZS-EPMC97974433589946610.1111/all.15457