biostudies-literatureVismans GFoundation TKI HorticultureChina Scholarship CouncilDutch Research Council (NWO)22473https://www.ebi.ac.uk/biostudies/studies/S-EPMC9797477biostudies-literatureUnknown12(1)Plants deposit photosynthetically-fixed carbon in the rhizosphere, the thin soil layer directly around the root, thereby creating a hospitable environment for microbes. To manage the inhabitants of this nutrient-rich environment, plant roots exude and dynamically adjust microbe-attracting and -repelling compounds to stimulate specific members of the microbiome. Previously, we demonstrated that foliar infection of Arabidopsis thaliana by the biotrophic downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa) leads to a disease-induced modification of the rhizosphere microbiome. Soil conditioned with Hpa-infected plants provided enhanced protection against foliar downy mildew infection in a subsequent population of plants, a phenomenon dubbed the soil-borne legacy (SBL). Here, we show thatScientific reportsCoumarin biosynthesis genes are required after foliar pathogen infection for the creation of a microbial soil-borne legacy that primes plants for SA-dependent defenses.PMC9797477024.004.014OCENW.GROOT.2019.0631605-106NWA.ID.17.04014219Berendsen RLPieterse CMJBakker PAHMvan Bentum SSchilder MSnoek BLDong LGoossens PBouwmeester HJSpooren JVismans GValls JThiombiano BPetriacq PSong YfalseCoumarin biosynthesis genes are required after foliar pathogen infection for the creation of a microbial soil-borne legacy that primes plants for SA-dependent defenses.Plants deposit photosynthetically-fixed carbon in the rhizosphere, the thin soil layer directly around the root, thereby creating a hospitable environment for microbes. To manage the inhabitants of this nutrient-rich environment, plant roots exude and dynamically adjust microbe-attracting and -repelling compounds to stimulate specific members of the microbiome. Previously, we demonstrated that foliar infection of Arabidopsis thaliana by the biotrophic downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa) leads to a disease-induced modification of the rhizosphere microbiome. Soil conditioned with Hpa-infected plants provided enhanced protection against foliar downy mildew infection in a subsequent population of plants, a phenomenon dubbed the soil-borne legacy (SBL). Here, we show that2022-01-01T00:00:00Z2022 Dec2025-04-22T17:59:42.175Z2025-02-19T03:30:32.085ZS-EPMC97974773657776410.1038/s41598-022-26551-x