{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Wang X"],"pubmed_abstract":["<i>Mucor circinelloides</i> WJ11, an oleaginous filamentous fungus, produces 36% lipid of its cell dry weight when cultured in a high C/N ratio medium, however, the yield of γ-linolenic acid (GLA) is insufficient to make it competitive with other plant sources. To increase the GLA content in <i>M. circinelloides</i> WJ11, this fungus was engineered by overexpression of its key genes such as Δ6-, Δ12-, and Δ9-desaturases involved in GLA production. Firstly, we tried to overexpress two Δ6-desaturase isozymes to determine which one played important role in GLA synthesis. Secondly, Δ6-and Δ12-desaturase were co-overexpressed to check whether linoleic acid (LA), the precursor for GLA synthesis, is a limiting factor or not. Moreover, we tried to explore the effects of simultaneous overexpression"],"journal":["Frontiers in microbiology"],"pagination":["1078157"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9797528"],"repository":["biostudies-literature"],"pubmed_title":["Simultaneous overexpression of ∆6-, ∆12- and ∆9-desaturases enhanced the production of γ-linolenic acid in <i>Mucor circinelloides</i> WJ11."],"pmcid":["PMC9797528"],"pubmed_authors":["Xue F","Yang J","Shah AM","Pang S","Li S","Sadaqat B","Wu C","Shi W","Wang X","Mohamed H","Song Y"],"additional_accession":[]},"is_claimable":false,"name":"Simultaneous overexpression of ∆6-, ∆12- and ∆9-desaturases enhanced the production of γ-linolenic acid in <i>Mucor circinelloides</i> WJ11.","description":"<i>Mucor circinelloides</i> WJ11, an oleaginous filamentous fungus, produces 36% lipid of its cell dry weight when cultured in a high C/N ratio medium, however, the yield of γ-linolenic acid (GLA) is insufficient to make it competitive with other plant sources. To increase the GLA content in <i>M. circinelloides</i> WJ11, this fungus was engineered by overexpression of its key genes such as Δ6-, Δ12-, and Δ9-desaturases involved in GLA production. Firstly, we tried to overexpress two Δ6-desaturase isozymes to determine which one played important role in GLA synthesis. Secondly, Δ6-and Δ12-desaturase were co-overexpressed to check whether linoleic acid (LA), the precursor for GLA synthesis, is a limiting factor or not. Moreover, we tried to explore the effects of simultaneous overexpression","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-04T12:02:03.945Z","creation":"2025-02-19T02:59:59.151Z"},"accession":"S-EPMC9797528","cross_references":{"pubmed":["36590442"],"doi":["10.3389/fmicb.2022.1078157"]}}