biostudies-literatureUnknown13Wang X<i>Mucor circinelloides</i> WJ11, an oleaginous filamentous fungus, produces 36% lipid of its cell dry weight when cultured in a high C/N ratio medium, however, the yield of γ-linolenic acid (GLA) is insufficient to make it competitive with other plant sources. To increase the GLA content in <i>M. circinelloides</i> WJ11, this fungus was engineered by overexpression of its key genes such as Δ6-, Δ12-, and Δ9-desaturases involved in GLA production. Firstly, we tried to overexpress two Δ6-desaturase isozymes to determine which one played important role in GLA synthesis. Secondly, Δ6-and Δ12-desaturase were co-overexpressed to check whether linoleic acid (LA), the precursor for GLA synthesis, is a limiting factor or not. Moreover, we tried to explore the effects of simultaneous overexpressionFrontiers in microbiology1078157https://www.ebi.ac.uk/biostudies/studies/S-EPMC9797528biostudies-literatureSimultaneous overexpression of ∆6-, ∆12- and ∆9-desaturases enhanced the production of γ-linolenic acid in <i>Mucor circinelloides</i> WJ11.PMC9797528Xue FYang JShah AMPang SLi SSadaqat BWu CShi WWang XMohamed HSong YfalseSimultaneous overexpression of ∆6-, ∆12- and ∆9-desaturases enhanced the production of γ-linolenic acid in <i>Mucor circinelloides</i> WJ11.<i>Mucor circinelloides</i> WJ11, an oleaginous filamentous fungus, produces 36% lipid of its cell dry weight when cultured in a high C/N ratio medium, however, the yield of γ-linolenic acid (GLA) is insufficient to make it competitive with other plant sources. To increase the GLA content in <i>M. circinelloides</i> WJ11, this fungus was engineered by overexpression of its key genes such as Δ6-, Δ12-, and Δ9-desaturases involved in GLA production. Firstly, we tried to overexpress two Δ6-desaturase isozymes to determine which one played important role in GLA synthesis. Secondly, Δ6-and Δ12-desaturase were co-overexpressed to check whether linoleic acid (LA), the precursor for GLA synthesis, is a limiting factor or not. Moreover, we tried to explore the effects of simultaneous overexpression2022-01-01T00:00:00Z20222025-04-04T12:02:03.945Z2025-02-19T02:59:59.151ZS-EPMC97975283659044210.3389/fmicb.2022.1078157