{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Morstein J"],"funding":["Division of Chemistry","National Institute of Neurological Disorders and Stroke","National Cancer Institute","NCI NIH HHS","NINDS NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["2945-2953"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9799063"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(10)"],"pubmed_abstract":["Photoswitchable lipids have emerged as attractive tools for the optical control of lipid bioactivity, metabolism, and biophysical properties. Their design is typically based on the incorporation of an azobenzene photoswitch into the hydrophobic lipid tail, which can be switched between its <i>trans</i>- and <i>cis</i>-form using two different wavelengths of light. While glycero- and sphingolipids have been successfully designed to be photoswitchable, isoprenoid lipids have not yet been investigated. Herein, we describe the development of photoswitchable analogs of an isoprenoid lipid and systematically assess their potential for the optical control of various steps in the isoprenylation processing pathway of CaaX proteins in <i>Saccharomyces cerevisiae</i>. One photoswitchable analog of farnesyl diphosphate (<b>AzoFPP-1</b>) allowed effective optical control of substrate prenylation by farnesyltransferase. The subsequent steps of isoprenylation processing (proteolysis by either Ste24 or Rce1 and carboxyl methylation by Ste14) were less affected by photoisomerization of the group introduced into the lipid moiety of the substrate a-factor, a mating pheromone from yeast. We assessed both proteolysis and methylation of the a-factor analogs <i>in vitro</i> and the bioactivity of a fully processed a-factor analog containing the photoswitch, exogenously added to cognate yeast cells. Combined, these data describe the first successful conversion of an isoprenoid lipid into a photolipid and suggest the utility of this approach for the optical control of protein prenylation."],"journal":["ACS chemical biology"],"pubmed_title":["Photoswitchable Isoprenoid Lipids Enable Optical Control of Peptide Lipidation."],"pmcid":["PMC9799063"],"funding_grant_id":["K00CA253758","R01NS108151","R01GM132606","R01 GM132606","K00 CA253758","R01 NS108151","R35 GM141853","NSF/CHE 1905204"],"pubmed_authors":["Hougland JL","Bader T","Schackmann J","Cardillo AL","Ashok S","Distefano MD","Morstein J","Hrycyna CA","Trauner DH"],"additional_accession":[]},"is_claimable":false,"name":"Photoswitchable Isoprenoid Lipids Enable Optical Control of Peptide Lipidation.","description":"Photoswitchable lipids have emerged as attractive tools for the optical control of lipid bioactivity, metabolism, and biophysical properties. Their design is typically based on the incorporation of an azobenzene photoswitch into the hydrophobic lipid tail, which can be switched between its <i>trans</i>- and <i>cis</i>-form using two different wavelengths of light. While glycero- and sphingolipids have been successfully designed to be photoswitchable, isoprenoid lipids have not yet been investigated. Herein, we describe the development of photoswitchable analogs of an isoprenoid lipid and systematically assess their potential for the optical control of various steps in the isoprenylation processing pathway of CaaX proteins in <i>Saccharomyces cerevisiae</i>. One photoswitchable analog of farnesyl diphosphate (<b>AzoFPP-1</b>) allowed effective optical control of substrate prenylation by farnesyltransferase. The subsequent steps of isoprenylation processing (proteolysis by either Ste24 or Rce1 and carboxyl methylation by Ste14) were less affected by photoisomerization of the group introduced into the lipid moiety of the substrate a-factor, a mating pheromone from yeast. We assessed both proteolysis and methylation of the a-factor analogs <i>in vitro</i> and the bioactivity of a fully processed a-factor analog containing the photoswitch, exogenously added to cognate yeast cells. Combined, these data describe the first successful conversion of an isoprenoid lipid into a photolipid and suggest the utility of this approach for the optical control of protein prenylation.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-05-28T02:15:37.651Z","creation":"2025-04-04T03:00:37.563Z"},"accession":"S-EPMC9799063","cross_references":{"pubmed":["36194691"],"doi":["10.1021/acschembio.2c00645"]}}