biostudies-literatureHuang CNatural Science Foundation of ShanghaiNational Natural Science Foundation of China102345https://www.ebi.ac.uk/biostudies/studies/S-EPMC9800190biostudies-literatureUnknown102(2)In 2018, a disease characterized by splenic hemorrhage and necrosis killed ducks in a duck farm in Guangxi province, China. A duck reovirus strain was isolated from the tissues of the dead ducks by inoculating duck embryos and BHK-21 cells. Electron microscopy of the cultured the isolate showed that the viral particles were nearly round in shape and approximately 70 nm in diameter, and they were designated DRV-GL18. Sequence analysis showed that the GL18 strain viral genome was 23,419 nt in length and had 10 dsRNA segments. Phylogenetic analysis of cDNA amplicons of segments encoding the protein σC which are outer capsid proteins showed that the isolate belongs to the branch of the epidemic strains of duck reovirus. The Recombination Detection Program (RDP) and SimPlot program analyses sugPoultry scienceIsolation and characterization of a duck reovirus strain from mature ducks in China.PMC98001903150206819ZR1468800U1703117Liu YHuang CLiu ZLiu JLi JHuang YChen ZHan JChen HfalseIsolation and characterization of a duck reovirus strain from mature ducks in China.In 2018, a disease characterized by splenic hemorrhage and necrosis killed ducks in a duck farm in Guangxi province, China. A duck reovirus strain was isolated from the tissues of the dead ducks by inoculating duck embryos and BHK-21 cells. Electron microscopy of the cultured the isolate showed that the viral particles were nearly round in shape and approximately 70 nm in diameter, and they were designated DRV-GL18. Sequence analysis showed that the GL18 strain viral genome was 23,419 nt in length and had 10 dsRNA segments. Phylogenetic analysis of cDNA amplicons of segments encoding the protein σC which are outer capsid proteins showed that the isolate belongs to the branch of the epidemic strains of duck reovirus. The Recombination Detection Program (RDP) and SimPlot program analyses sug2023-01-01T00:00:00Z2023 Feb2026-05-28T12:54:40.83Z2025-02-18T22:34:31.144ZS-EPMC98001903657187310.1016/j.psj.2022.102345